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ERK1 / ERK2 Mouse Monoclonal Antibody [Clone ID: SB46b]

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SKU
AM08205BT-N
ERK1 / ERK2 mouse monoclonal antibody, clone SB46b, Biotin
Size:
500 ug
Conjugation:
Biotin HRP Unconjugated
  $445.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name SB46b
Application IP, WB
Recommended Dilution Western Blot.
Predicted Mol. Weight: 42 and 44 kDa.
Immunoprecipitation.
Reactivity Human
Antibody Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Human recombinant Erk1 and Erk2.
Specificity This antibody precipitates ~42 kDa and ~44 kDa bands, corresponding to Erk1 and Erk2, respectively.
Buffer PBS containing 0.09% Sodium Azide as preservative.
Label: Biotin
State: Liquid purified Ig fraction.
Concentration lot specific
Conjugation Biotin
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Background Erk1 and Erk2 are closely related mitogen activated protein (MAP) kinases which are activated by many growth factors, mitogens and differentiation-promoting agents via a protein kinase cascade. Also known as extracellular signal-regulated kinase 1 and 2, p44/p42 MAP kinases, microtubule-associated protein-2 kinases, myelin basic protein (MBP) kinases or EGF receptor T669 (Ert) kinases. (Ref.1-4) Erk1 and Erk2 are ubiquitous and abundant, although their relative abundance in specific tissues may vary. The two kinases are nearly 85% identical and have higher identity in the core regions involved in substrate binding. (Ref.4) Erk1 and Erk2 are activated approximately 1000-fold by phosphorylation within a Thr-Glu-Tyr motif in the activation loop on both threonine and tyrosine residues by Mek1 and Mek2. (Ref.4,5)
Both sites must be phosphorylated for maximum activity. (Ref.1-4) These kinases in turn phosphorylate a variety of different substrates.
Erk1 and Erk2 are found in the cytoplasm and are translocated to the nucleus upon activation. Erk1 and Erk2 target membrane proteins, cytosolic proteins, such as downstream kinases, and cytoskeletal proteins and nuclear proteins, such as transcription factors. Many of these substrates are important regulatory proteins. Erk1 and Erk2 represent proximal kinases in the classical kinase pathway which links growth and differentiation signals at the cell surface (through tyrosine kinases) with transcription in the nucleus. (Ref.1-3)
Synonyms ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK
Reference Data
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Documents
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file-alt Citations

Pharmaceutical inhibition of AXL suppresses tumor growth and invasion of esophageal squamous cell carcinoma
Han, Wang, Ge et al
Exp Ther Med (2020) 20 (5), 41 DOI: 10.3892/etm.2020.9169
Cited by 6
AI Summary: OriGene products, specifically the AXL antibody (Cat# TA301606) and AKT antibody (Cat# TA312032), were used in this study to detect the expression and signaling activity of AXL and AKT proteins via western blotting, helping to demonstrate the inhibitory effects of R428 on ESCC tumor cell proliferation and invasion.
FigureFigureFigure
Minimal contribution of ERK1/2-MAPK signalling towards the maintenance of oncogenic GNAQQ209P-driven uveal melanomas in zebrafish
Mouti, Dee, Coupland et al
Oncotarget (2016)
Cited by 36
AI Summary: OriGene contributed the human Myc-DDK tagged GNAQ cDNA cloned into the pCMV6 vector, which was used for transient transfection experiments to investigate the role of GNAQ mutations in uveal melanoma in this study.
Snippet: Transient transfection Human Myc-DDK tagged GNAQ cDNA cloned into mammalian pCMV6 vector was purchased from Origene. To generate mutant clones, wild-type GNAQ coding sequence was mutagenized using Quickchange II XL site-directed mutagenesis kit (Stratagene). Mutagenic primers were designed using NEBase changer software (http://nebasechanger.neb.com), and were as follows: GNAQQ209P_Fwd: 5’-GATGTAGGGGGCCCAAGGTCAGAGAG-3’ GNAQQ209L_Fwd: 5’-GATGTAGGGGGCCTAAGGTCAGAGAG-3’, and GNAQQ209P/L_Rev: 5’-CTTCTCTCTGACCTTTGGCCCCCTACATCG-3’ One day before transfection, cells were seeded at a density of 2.5 x 105 per well of a 12 well plate format under complete growth conditions. …
IHC
The genomic landscape of response to EGFR blockade in colorectal cancer
Bertotti, Papp, Jones et al
Nature (2015) 526 (7572), 263-7 DOI: 10.1038/nature14969
Cited by 501
AI Summary: OriGene contributed to this study by providing custom-cloned lentiviral vectors expressing EGFR G465E and MAP2K1 K57N (PS100069), as well as the mouse anti-DDK (4C5) antibody, which were used to investigate mechanisms of resistance and sensitivity to EGFR blockade in colorectal cancer.
Snippet: … Cell lines were authenticated for genetic identity by short tandem repeat profiling (Cell ID, Promega) and routinely PCR-tested for mycoplasma contamination (Minerva Biolabs). EGFR G465E and MAP2K1 K57N in the PS100069 lentiviral vectors were custom-cloned by and purchased from OriGene. …
The Kinase Activity-deficient Isoform of Araf Antagonizes Ras/MAPK Signaling in the Zebrafish Embryo
Xiong, Liu, Meng
J. Biol. Chem. (2015)
AI Summary: In this study, the anti-ERK1 antibody from OriGene was used to detect ERK1 protein levels, contributing to the analysis of MAPK pathway activity in zebrafish embryos.
Snippet: The used primary antibodies were: anti-Myc and anti-HA from Santa Cruz;anti-Flag from Sigma;anti-Smad2/3 ， anti-phospho-Smad2 （ Ser465/467 ） and anti-phospho-ERK1/2 (Thr202/Tyr204) from Cell Signaling Technology;anti-ERK1 from OriGene;... Cite SaveSaving
WB
Novel cross talk between IGF-IR and DDR1 regulates IGF-IR trafficking, signaling and biological responses
Malaguarnera, Nicolosi, Sacco et al
Oncotarget (2015)
Snippet: terials IGF-I was purchased from Prepotech (Rocky Hill, NJ);Bovine Serum Albumin (BSA), fibronectin, collagen Type I and IV from Sigma Aldrich (St. Louis, MO);Metafectene PRO from Biontex Laboratories GmbH (Germany);lipofectamine 2000, lipofectamine RNAiMax, Opti-MEM, fetal calf serum (FCS), Geneticin (G-418) from Life Technologies, Inc. Laboratories (Paisley, UK). Constructs encoding either an em...
Showing 1-5 of 5 papers.
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