Pirh2 (RCHY1) Mouse Monoclonal Antibody [Clone ID: 1H10]

SKU AM06384SU-N

Pirh2 (RCHY1) mouse monoclonal antibody, clone 1H10, Ascites

Product Data

• Clone Name: 1H10
• Application: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000. ; Western Blot: 1/500-1/2000. ; Immunofluorescence: 1/200-1/1000.; Flow Cytometry: 1/200-1/400.; Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
• Reactivity: Human, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human Pirh2 expressed in E. Coli.
• Specificity: This antibody recognizes Ring Finger and CHY Zinc Finger Domain Containing 1 (RCHY1).
• Buffer: State: Ascites; State: Ascitic fluid; Preservative: 0.03% Sodium Azide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Predicted Protein Size: 30 kDa; 60 kDa (homodimer)
• Gene Name: ring finger and CHY zinc finger domain containing 1
• Database Link:
  Q96PM5
• Background: Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
• Synonyms: ARNIP, CHIMP, RNF199, ZNF363

Reference Data