Islet 1 (ISL1) Mouse Monoclonal Antibody [Clone ID: 1H9]

CAT#: AM06335SU-N

Islet 1 (ISL1) mouse monoclonal antibody, clone 1H9, Ascites

Product Images

Western blot analysis using ISL1 mouse mAb against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate (Lane 1).

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  Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and cervical carcinoma (right), showing nuclear localization using ISL1 antibody Cat.-No AM06335SU-N with DAB staining.

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  Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 antibody Cat.-No AM06335SU-N (green). Blue: DRAQ5 fluorescent DNA dye.

Specifications

Product Data

• Clone Name: 1H9
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human ISL1 expressed in E. Coli.
• Specificity: Recognizes ISL1 (ISL1 transcription factor).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 39 kDa
• Gene Name: ISL LIM homeobox 1
• Database Link:
  Entrez Gene 3670 Human
  UniProt ID P61371
• Background: ISL1 (ISL1 transcription factor, LIM/homeodomain) is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. It is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry. Mutations in this gene have been associated with maturity-onset diabetes of the young.
• Synonyms: Isl-1; islet-1; ISLET1