GFP Mouse Monoclonal Antibody [Clone ID: N/K]
Specifications
| Product Data | |
| Clone Name | N/K |
|---|---|
| Application | ELISA, IHC, WB |
| Recommended Dilution | ELISA: 1/1000-1/2000. Western blot: 1/2000-1/5000. Immunohistochemistry on Frozen Sections: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/500-1/1000. |
| Reactivity | GFP |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | GFP-BSA conjugate |
| Specificity | This antibody recognises various forms of Green Fluorescent Protein including eGFP and those forms found in vectors supplied by Clontech and Invitrogen. The antibody does not cross react with RFP. Cross reactivity with YFP has not been tested. |
| Buffer | Phosphate buffered saline State: Purified State: Liquid purified IgG fraction Stabilizer: BSA |
| Conjugation | Unconjugated |
| Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Database Link | |
| Background | Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. |
| Synonyms | Green fluorescent protein, GFP-Tag |
| Reference Data | |
Reviews
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Citations
In situ imaging of quantum dot-AZD4547 conjugates for tracking the dynamic behavior of fibroblast growth factor receptor 3
Hwang, Kim, Yoon et al
Int J Nanomedicine (2017) 12, 5345-5357 DOI: 10.2147/IJN.S141595
Hwang, Kim, Yoon et al
Int J Nanomedicine (2017) 12, 5345-5357 DOI: 10.2147/IJN.S141595
AI Summary: OriGene contributed Turbo GFP tagged FGFR3 plasmid DNA, which was used to overexpress fluorescently labeled FGFR3 in HeLa cells for imaging and tracking FGFR3-inhibitor interactions in the study.
Snippet: Transfection was performed by using Turbo GFP tagged FGFR3 plasmid DNA (Origene, Rockvile, MD, USA) and Lipofectamine 2000 reagent (Thermo Fisher Scientific). HeLa cells were plated in an 8-well chamber slide at 2×10 4 cells/well. ... All 3 versions Cite SaveSaving
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