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Cd68 Mouse Monoclonal Antibody [Clone ID: ED1]

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SKU
AM03211PU-N
Cd68 mouse monoclonal antibody, clone ED1, Aff - Purified
Size:
250 ug
Conjugation:
Unconjugated
  $1,085.00
2 Weeks*
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View other "Cd68 or NP_001026808.1" antibodies

Specifications
Specifications
Product Data
Clone Name ED1
Application FC, IHC, IP, WB
Recommended Dilution Immunohistochemistry on Paraffin Sections: 10 µg/ml (1/100), no antigen retrieval required.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml (1/1000-1/2000).
Western Blot.
Immunoprecipitation.
FACS (preferably on permeabilized cells).
Suggested Positive Control: Rat spleen.
Reactivity Rat
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Rat spleen cells.
Specificity This Monoclonal Antibody ED1 is useful for detecting Rat monocytes and macrophages and isolated dendritic (veiled) cells in the blood.
The antibody recognises a single chain glycoprotein of 90-100kDa. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 has many characteristics in common with Mouse macrosialin and Human CD68.
Antigen, Epitope: CD68; ED1 recognises a 92kD cytoplasmic protein. The epitope has not been further characterized.
Antigen Distribution: The antigen is found on 90% of monocytes in the peripheral blood. It is also expressed by 98% of isolated dendritic (veiled) cells.
Buffer PBS, pH 7.2
State: Aff - Purified
State: Liquid purified Ig fraction
Preservative: 0.09% Sodium Azide
Concentration 1.0 mg/ml
Purification Affinity Chromatography
Conjugation Unconjugated
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Gene Name Cd68 molecule
Database Link
Background The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
Synonyms Gp110, Macrosialin, Macrophage marker
Note Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
(The whole procedure is performed at room temperature)

1. Wash in PBS.
2. Block endogenous peroxidase.
3. Wash in PBS.
4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber.
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber.
6. Wash in PBS.
7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to rat) for 1h in a humid chamber.
8. Wash in PBS.
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS.
11. Counterstain with Mayer's hemalum.

Protocol with formalin-fixed, paraffin-embedded sections:
(The whole procedure is performed at room temperature)

1. Deparaffinize and rehydrate tissue section.
2. Block endogenous peroxidase.
3. Wash in PBS.
4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber.
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber.
6. Wash in PBS.
7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to rat) for 1h in a humid chamber.
8. Wash in PBS.
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS.
11. Counterstain with Mayer's hemalum.
Reference Data
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Documents
Resources

file-alt Citations

Isolated Langerhans cell histiocytosis of the stomach: a case report and literature review
Yan, Zhou, Gao et al
Int J Clin Exp Pathol (2018) 11 (12), 5962-5968
AI Summary: OriGene products contributed to this paper by providing the anti-CD1a antibody (OriGene, TA802306) used for immunohistochemical staining to help confirm the diagnosis of Langerhans cell histiocytosis in the gastric tissue.
Tumor necrosis factor antagonists in the treatment of multicentric reticulohistiocytosis: Current clinical evidence
Zhao, Wu, Wu et al
Mol Med Rep (2016)
Cited by 19
AI Summary: In this study, OriGene's normal donkey serum (NDS) was used to block nonspecific reactions during immunohistochemical staining of biopsy samples, aiding in the diagnosis of multicentric reticulohistiocytosis.
Snippet: tissue penetration by antibodies, sections were incubated with ethanol for 30 min and rinsed with phosphate‑buffered saline (PBS) 3 times for 5 min then blocked to prevent nonspecific primary antibody reactions with 10% normal donkey serum (NDS;OriGene ... Cite SaveSaving
IHC
Showing 1-2 of 2 papers.
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