CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: DJ130c]
$645.00
5 Days*
Specifications
| Product Data | |
| Clone Name | DJ130c |
|---|---|
| Application | FC, IHC |
| Recommended Dilution | Flow Cytometry: Use 10 µl of 1/20-1/200 diluted antibody to label 1x106 cells in 100 µl. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human tonsil. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | CD34 antigen |
| Specificity | This antibody recognizes Human CD16, a 50-65kD cell surface molecule. It recognizes all polymorphonuclear cells irrespective on their NA phenotype. Studies show that the DJ130c antibody recognizes an epitope in the first membrane-distal domain of CD16 and recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from Rhesus macaques, Macaca mulatta (Xu et al. 2012). |
| Buffer | PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | Fc fragment of IgG receptor IIIa |
| Database Link | |
| Background | CD16 which is the low affinity receptor for IgG (FcRIII), exists as a transmembranous form (Fc gammaRIIIA, or CD16a) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16b). CD16a is expressed by NK cells, some T cells, and macrophages, whereas CD16b is primarily expressed by granulocytes. In addition, CD16b exists as two allelic variants NA1 and NA2. |
| Synonyms | FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2 |
| Reference Data | |
| Protein Families | ES Cell Differentiation/IPS, Secreted Protein, Transmembrane |
| Protein Pathways | Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity, Systemic lupus erythematosus |
Reviews
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.