LOC396098 Mouse Monoclonal Antibody [Clone ID: L22]

CAT#: AM01028FC-N

LOC396098 mouse monoclonal antibody, clone L22, FITC

Specifications

Product Data

• Clone Name: L22
• Applications: FC
• Recommended Dilution: Flow Cytometry: 1/20 - 1/80; Use 10ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request.
• Reactivities: Chicken
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Chicken Bursa cells
• Specificity: MCA2170F recognises the 'a' allele of the polymorphic cell surface antigen Bu-1.
• Formulation: BPS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Storage: Store at +4oC or at -20oC if preferred. This product should be stored undiluted.
  Avoid repeated freezing and thawing. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: one year from despatch.
• Gene Name: B6.1
• Database Link:
  Entrez Gene 396098 Chicken
  UniProt ID Q90746
• Background: Bu-1a is a 70kD alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development. Weak cross-reactivity with Bu-1b may be seen at higher antibody levels. This may be removed by appropriate titration.
  Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line.
• Synonyms: B-Cell Marker