AKT2 (specific) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 8B7]

CAT#: AM00109PU-N

AKT2 (specific) (incl. pos. control) mouse monoclonal antibody, clone 8B7, Purified

Product Images

Detection of endogenous akt2 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab PKB-8B7 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2:SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF7 Lane 6: MDA-MB-231 Lane 7: T47D

Specifications

Product Data

• Clone Name: 8B7
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 0.1 µg/ml.
  Immunohistochemistry.
  Western Blot: 1 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Included Positive Control: Cell lysate from untreated HepG2 cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to KLH.
  Epitope: aa 107-123
• Specificity: This antibody specifically recognizes the beta isoform of PKB (PKBβ/Akt2).
• Formulation: 1 ml, 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Thiophilic Adsorption and Size Exclusion Chromatography.
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Gene Name: AKT serine/threonine kinase 2
• Database Link:
  Entrez Gene 208 Human
  UniProt ID P31751
• Background: Akt, protein kinase B (PKB), is a serine/threonine kinase, which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.
  AKT2 encodes a RAC/AKT-type protein kinase that contains a N-terminal pleckstrin-homology (PH) domain and a central catalytic domain closely related to both cAMP-dependent protein kinase and protein kinase C. The protein is a member of PI3K-mediated signalling pathways associated with the regulation of proliferation, survival, protein synthesis, and metabolism. It is activated by a variety of growth factors. AKT2 has been shown to be transcriptionally regulated by MyoD and to activate MyoD-myocyte enhancer binding factor-2 (MEF2) transactivation activity during muscle differentiation. Glycogen synthase kinase 3 (GSK-3) also has been shown to be a downstream target of AKT2. The AKT2 gene is one of the human homologues of v-akt, the transduced oncogene of the AKT8 virus, which induces lymphomas in mice. It has been implicated in breast, ovarian, and pancreatic cancers.
• Synonyms: RAC-PK-beta, Protein kinase Akt-2, Protein kinase B beta
• Note: Molecular Weight: 60 kDa
  
  Protocol: Positive Control Cell Lysate: HepG2 untreated.
  
  Format: Lyophilized cell lysate from serum starved HepG2 cells.
  
  Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  Aliquote and store frozen, avoid repeated freezing and thawing.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells.
  Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  
  Note: The lyophilized cell lysates contain SDS and are NOT recommended for applications with native proteins such as immunoprecipitation.