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GAPDH Goat Polyclonal Antibody

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SKU
AB0067-200
Goat Polyclonal Anti-GAPDH Antibody
Size:
400 ug
Conjugation:
Unconjugated
  $485.00
In Stock*
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View other "GAPDH or NM_002046" antibodies

Specifications
Specifications
Product Data
Application IF, WB
Recommended Dilution WB 1:500-1:2,000; IF 1:50-1:250; IHC (paraffin) 1:200-1:1,000; IHC (frozen) 1:200-1:1,000
Reactivity Canine, Human, Monkey, Mouse, Rat
Antibody Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.
Specificity Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U- 118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.
Buffer Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Concentration 2mg/ml
Purification epitope affinity purified
Conjugation Unconjugated
Shipping Blue Ice
Gene Name glyceraldehyde-3-phosphate dehydrogenase
Database Link
Background Glyceraldehyde-3-phosphate dehydrogenase
Synonyms G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432
Note Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.
Reference Data
Protein Categories Cytokines, Intracellular Proteins
Protein Families ES Cell Differentiation/IPS
Protein Pathways Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways
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Documents
Resources

file-alt Citations

Lipocalin-2 exerts pro-atherosclerotic effects as evidenced by in vitro and in vivo experiments
Shibata, Sato, Shirai et al
Heart Vessels (2020)
Cited by 43
AI Summary: In this study, the GAPDH antibody from Acris-OriGene Technologies was used as a loading control in Western blot experiments to assess protein expression changes related to the pro-atherosclerotic effects of Lipocalin-2.
Snippet: Lipocalin-2 (LCN2), a multiple bioactive hormone particularly expressed in adipose tissue, neutrophils, and macrophages, is known to exhibit anti-microbial.;signal-regulated kinase 1/2 (ERK1/2;dilution 1:1000), phosphorylated p38 (dilution 1:1000;Cell Signaling Technology, Danvers, MA), phosphoinositide 3-kinase (PI3K;dilution 1:1000;Abcam, Cambridge, UK), GAPDH (dilution 1:5000;Acris-OriGene Tech...
WB
Empowering human cardiac progenitor cells by P2Y14 nucleotide receptor overexpression
Khalafalla, Kayani, Kassab et al
J. Physiol. (Lond.) (2017)
AI Summary: OriGene contributed to this study by providing the lentiviral plasmids encoding P2Y14R fused to mGFP and monomeric GFP (mGFP), which were used to overexpress P2Y14 receptor in human cardiac progenitor cells and assess its effects on cell function.
Snippet: in a 6-well plate (30,000 cells/well) overnight then transduced with lentiviral particles encoding monomeric green fluorescent protein (2 MOI;hCPC-mGFP) or P2Y14R fused to mGFP (0.2 MOI;hCPC-Y14) (lentiviral plasmids were purchased from Origene - P2Y14R ...;... in a 6-well plate (30,000 cells/well) overnight then transduced with lentiviral particles encoding monomeric green fluorescent protein (2 ...
Protective Effect of a GLP-1 Analog on Ischemia-Reperfusion Induced Blood-Retinal Barrier Breakdown and Inflammation
Gonçalves, Lin, Muthusamy et al
Invest Ophthalmol Vis Sci (2016) 57 (6), 2584-92 DOI: 10.1167/iovs.15-19006
Cited by 69
AI Summary: In this study, OriGene's qPCR primers (catalog #s: NM_012922, NM_017008, NM_013093) were used to measure retinal inflammatory gene expression, enabling the authors to assess the anti-inflammatory effects of the GLP-1 analog Exendin-4 after ischemia-reperfusion injury.
FigureFigureFigure
RAL-1 controls multivesicular body biogenesis and exosome secretion
Hyenne, Apaydin, Rodriguez et al
J Cell Biol (2015) 211 (1), 27-37 DOI: 10.1083/jcb.201504136
Cited by 232
AI Summary: OriGene products contributed to this study by providing the RalA and RalB shRNA plasmids, which were used to knock down these genes in mammalian cells to assess their roles in exosome secretion.
FigureFigureFigure
CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited
Helsby, Leader, Fenn et al
BMC Cell Biol (2014) 15, 6 DOI: 10.1186/1471-2121-15-6
Cited by 50
AI Summary: In the paper "CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited," OriGene products such as antibodies from OriGene were included in the CiteAb database, allowing researchers to identify and select OriGene antibodies that have been validated and cited in peer-reviewed publications.
FigureFigureFigure
Showing 1-5 of 5 papers.
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