GAPDH Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | IF, WB |
|---|---|
| Recommended Dilution | WB 1:500-1:5,000; IF 1:50-1:250; IHC (paraffin) 1:200-1:1,000; IHC (frozen) 1:200-1:1,000 |
| Reactivity | Canine, Human, Monkey, Mouse, Rat, Zebrafish |
| Antibody Host | Goat |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli. |
| Specificity | Detects a band of 37 kDa by Western blot in the following Human (293A, HMEC-1, U- 118, HaCat), Rat (TR-iBRB), Mouse (AtT-20, Hepa), Canine (D17) and Monkey (COS-7) whole cell lysates. |
| Buffer | Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. |
| Concentration | 2mg/ml |
| Purification | epitope affinity purified |
| Conjugation | Unconjugated |
| Shipping | Blue Ice |
| Gene Name | glyceraldehyde-3-phosphate dehydrogenase |
| Database Link | |
| Background | Glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms | G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432 |
| Note | Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates. |
| Reference Data | |
| Protein Categories | Cytokines, Intracellular Proteins |
| Protein Families | ES Cell Differentiation/IPS |
| Protein Pathways | Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways |
Reviews
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Citations
Androgen receptor activation inhibits endothelial cell migration in vitro and angiogenesis in vivo
Huo, Yang, Ke et al
Eur J Cell Biol (2024) 103 (4), 151456 DOI: 10.1016/j.ejcb.2024.151456
Huo, Yang, Ke et al
Eur J Cell Biol (2024) 103 (4), 151456 DOI: 10.1016/j.ejcb.2024.151456
Cited by 4
AI Summary: OriGene’s GAPDH goat polyclonal antibody (CAT#: AB0049-200) was used as a loading control in Western blot experiments to assess protein expression in this study on androgen receptor-mediated effects on endothelial cell migration and angiogenesis.
Snippet: (#9698) were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). GAPDH goat polyclonal antibody(CAT#: AB0049–200) were purchased from ORIGENE (Rockville, USA). anti-PR CAT #MA1–410 and anti-ERα CAT #MA1–310 were purchased from (Thermo Fisher Scientific, USA). The antibodies were used at a concentration of 1:1000 dilution ex
β-Endorphin Mediates the Development and Instability of Atherosclerotic Plaques
Okano, Sato, Shirai et al
Int J Endocrinol (2020) 2020, 4139093 DOI: 10.1155/2020/4139093
Okano, Sato, Shirai et al
Int J Endocrinol (2020) 2020, 4139093 DOI: 10.1155/2020/4139093
Cited by 15
AI Summary: In this study, OriGene’s anti-OPRM1 antibody (TA326433) was used to detect μ-opioid receptor expression, contributing to the investigation of β-endorphin’s role in atherosclerosis development and plaque instability.
Extracellular vesicles derived from Hepatitis-D Virus infected cells induce a proinflammatory cytokine response in human peripheral blood mononuclear cells and macrophages
Jung, Altstetter, Wilsch et al
Matters (2020)
Jung, Altstetter, Wilsch et al
Matters (2020)
Snippet: am (Cambridge, UK) and anti-CD63 antibodies were from SBI (Palo Alto, California). Cellular proteins were stained using anti-Calnexin (BD Biosciences, San Jose, California) and anti-GAPDH antibodies (Acris Antibodies, Herford, Germany). Secondary antibodies (anti-mouse IgG Peroxidase and anti-rabbit IgG Peroxidase) were purchased from Sigma-Aldrich (St. Louis, Missouri). EVs were removed from 1:2
Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport
Robinson, Evans, Briggs et al
J Cell Sci (2017) 130 (12), 2056-2065 DOI: 10.1242/jcs.186064
Robinson, Evans, Briggs et al
J Cell Sci (2017) 130 (12), 2056-2065 DOI: 10.1242/jcs.186064
Cited by 17
AI Summary: OriGene products contributed to this study by providing the human KIF5B (RC210971) and RAB1A (RC202753) cDNA clones, which were used to investigate the roles of Kif5b and Rab1a in melanosome transport.
Hyperglycemia-induced degradation of HIF-1α contributes to impaired response of cardiomyocytes to hypoxia
Ramalho, Toscano, Pereira et al
Rev Port Cardiol (2017) 36 (5), 367-373 DOI: 10.1016/j.repc.2016.09.018
Ramalho, Toscano, Pereira et al
Rev Port Cardiol (2017) 36 (5), 367-373 DOI: 10.1016/j.repc.2016.09.018
Cited by 18
AI Summary: In this study, OriGene’s mouse HIF-1α antibody (Cat# TA500145) was used for western blot analysis to assess HIF-1α protein levels in cardiomyocytes under various conditions, helping to elucidate the role of hyperglycemia-induced degradation of HIF-1α in impaired hypoxic response.
K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy
Ferreira, Soares, Ramalho et al
Sci Rep (2015) 5, 10210 DOI: 10.1038/srep10210
Ferreira, Soares, Ramalho et al
Sci Rep (2015) 5, 10210 DOI: 10.1038/srep10210
Cited by 99
AI Summary: OriGene products, specifically the HIF1A cDNA clone (Cat# SC119516) and HIF1A shRNA plasmid (Cat# TL312590), were used in this study to manipulate HIF1A expression and investigate its degradation via chaperone-mediated autophagy.
Showing 1-6 of 8 papers.
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