RAB27A Goat Polyclonal Antibody

SKU
AB0023-200
Goat Polyclonal Anti-Rab27a Antibody
  $485.00
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Specifications
Specifications
Product Data
Application IHC, WB
Recommended Dilution WB 1:250-1:2,000; IF 1:50-1:200; IHC (paraffin) 1:50-1:400; IHC (frozen) 1:50-1:400
Reactivity Canine, Human, Monkey, Mouse, Rat
Antibody Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a produced in E. coli.
Specificity Detects Rab27a by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a.
Buffer Polyclonal antibody supplied as a 200 µl (1 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Concentration 1 mg/ml
Purification epitope affinity purified
Conjugation Unconjugated
Shipping Blue Ice
Gene Name RAB27A, member RAS oncogene family
Database Link
Background RAB27A, member RAS oncogene family
Synonyms GS2; HsT18676; MGC117246; Rab-27; RAB27; RAM
Note Detects Rab27a by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a. This antibody also recognizes Rab27b protein.
Reference Data
Protein Categories Immune system diseases, Intracellular Proteins
Protein Families Druggable Genome
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Documents
Resources

file-alt Citations

Alteration of Cytoskeleton Morphology and Gene Expression in Human Breast Cancer Cells under Simulated Microgravity
Strube, Infanger, Wehland et al
Cell J (2020) 22 (1), 106-114 DOI: 10.22074/cellj.2020.6537
Cited by 23
AI Summary: OriGene products, specifically rabbit polyclonal antibodies against vimentin, RhoA, Her2, RAB27A, and MAPK1, were used in this study to detect and confirm protein expression changes in human breast cancer cells under simulated microgravity.
Snippet: in blocking reagent with following dilutions: rabbit polyclonal anti-vimentin (1:2000), rabbit polyclonal anti- RhoA (1:500), rabbit polyclonal anti-Her2 (1:1000), rabbit polyclonal anti-RAB27A (1:1000), rabbit polyclonal anti- MAPK1 (1:1000), all obtained from Origene, USA, as
FigureFigureFigure
WB
Isolation of Intact and Functional Melanosomes from the Retinal Pigment Epithelium
Pelkonen, Reinisalo, Morin-Picardat et al
PLoS One (2016) 11 (8), e0160352 DOI: 10.1371/journal.pone.0160352
Cited by 23
AI Summary: In this paper, OriGene’s mouse anti-GAPDH monoclonal antibody (Cat# TA-08) was used for immunoblotting to confirm the purity of isolated melanosomal fractions by detecting potential contamination from other cellular components.
FigureFigureFigure
Rab27a GTPase modulates L-type Ca2+ channel function via interaction with the II-III linker of CaV1.3 subunit
Reichhart, Markowski, Ishiyama et al
Cell Signal (2015) 27 (11), 2231-40 DOI: 10.1016/j.cellsig.2015.07.023
Cited by 13
AI Summary: In this study, OriGene products—specifically, the human Rab27a Myc-DDK tagged expression plasmid (OriGene, RC200176) and the human CaV1.3 (CACNA1D) Myc-DDK tagged expression plasmid (OriGene, RC213838)—were used to heterologously express Rab27a and CaV1.3 in cell lines, enabling the investigation of their interaction and functional effects on L-type Ca2+ channel activity.
Showing 1-3 of 3 papers.
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