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beta Actin (ACTB) Goat Polyclonal Antibody

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SKU
AB0145-200
Goat Polyclonal Anti-beta-Actin Antibody
Size:
600 ug
Conjugation:
Unconjugated
  $485.00
In Stock*
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View other "beta Actin or NM_001101" antibodies

Specifications
Specifications
Product Data
Application IF, WB
Recommended Dilution WB 1:250-1:2,000; IF 1-50-1:250
Reactivity Canine, Human, Monkey, Mouse, Rat
Antibody Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Purified recombinant peptide derived from within residues 100 aa to the N-terminus of human beta-Actin produced in E. coli.
Specificity Detects a band of approximately 42 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC- 1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.
Buffer Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Concentration 3mg/ml
Purification epitope affinity purified
Conjugation Unconjugated
Shipping Blue Ice
Gene Name actin, beta
Database Link
Synonyms Beta-actin; PS1TP5BP1
Note Detects a band of approximately 42 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.
Reference Data
Protein Categories ADC Targets, Intracellular Proteins
Protein Families ES Cell Differentiation/IPS
Protein Pathways Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis
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Documents
Resources

file-alt Citations

Human pancreatic α-cell heterogeneity and trajectory inference analyses reveal SMOC1 as a β-cell dedifferentiation gene
Kang, Varela, Oh et al
Nat Commun (2025) 16 (1), 8434 DOI: 10.1038/s41467-025-62670-5
Cited by 9
AI Summary: OriGene’s anti-actin antibody (Cat# AB0145-200) was used for Western blotting as a loading control to assess protein expression in human islet extracts in this study of β-cell dedifferentiation.
Snippet: human islets and SMOC1 immunolabeling of human pancreas Human islet protein extracts were analyzed by Western blotting with antibodies against SMOC1 (Abcam, Cat# ab313569, dilution:1:1000) and actin (Origene, Rockville, MD, Cat#AB0145-200, dilution:1:1000)80. Human pancreas specimens were fixed for 24 h at 4 °C in 4% paraformaldehyde and then washed with PBS and placed in 70% ethanol until pa
FigureFigureFigure
WB
Effects of oxidized low-density lipoprotein on differentiation of mouse neural progenitor cells into neural cells
Ishizuka, Nagata, Nomura-Takahashi et al
Eur. J. Pharmacol. (2020) 888, 173456 DOI: 10.1016/j.ejphar.2020.173456
Cited by 3
AI Summary: In this study, OriGene's mouse anti-NeuN monoclonal antibody (Cat# TA336140) was used to assess neuronal differentiation of mouse neural progenitor cells by immunofluorescence staining and Western blot analysis.
Snippet: Next, the undifferentiated NPCs were incubated with mouse anti-PSA-NCAM antibody (1: 50;Miltenyi Biotec) or rabbit anti-Nestin antibody (1: 100;Acris Antibodies GmbH, Herford, Germany) in PBS containing 2.5% FBS at 4 °C overnight;After blocking, the membranes of the NPC extracts were incubated with a 1: 2,000 dilution of monoclonal rabbit anti-ox-LDL receptor-1 (LOX-1) antibody (Novus Biologicals)...
WB IF
Showing 1-2 of 2 papers.
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