OriGene's HotStart PCR Kit is supplied with a high fidelity HotStart DNA polymerase, 5X HF Buffer, 2.5X GC Buffer, and dNTPs. The HotStart DNA polymerase has a strong 3'-5' exonuclease activity (proofreading activity), which results in its extreme high fidelity, 10-15 times higher that Taq DNA polymerase and 6 times higher than Pyrococcus furiosus DNA polymerase. The antibody-mediated hot-start feature significantly inhibits non-specific amplifications at room temperature.
Key Features and Benefits
- High Fidelity: the HotStart DNA polymerase contains recombinant enhancement that strengthens the 3'-5' exonuclease activity.
- Efficient: Even problematic of GC-rich templates can be amplified using the 2.5X GC buffer provided in the kit.
- Specific: The antibody-mediated hot-start enzyme remains completely inactive during PCR set-up to prevent non-specific amplification.
|This HotStart PCR kit (2X) is ideal for high fidelity PCR with excellent processivity. The DNA polymerase contains a recombinant synthesis enhancement domain to increase fidelity and extension speed.
|cDNA Clone Resources