BXRF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101075

Myc-DDK-tagged ORF clone of viral ORF for BXRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129498

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BXRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101075 represents NCBI reference of YP_001129498 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCAACACGGGGCCTTTGTGCACTCTCTACTCATGATCTCGCCAAATTTCACAGCCTGCCTCCCG
  CCCGGAAAGCAGCAGGTAAGAGGGCACACCTCCGGTGTTACTCAAAGCTCCTGTCACTGAAATCTTGGGA
  GCAACTTGCAAGTTTCCTCTCTCTGCCCCCCGGCCCTACCTTTACCGACTTTAGACTTTTCTTTGAGGTA
  ACCCTCGGCAGGCGAATTGCAGATTGCGTTGTCGTCGCTCTCCAACCATCCCCTCGCTGCTACATCGTTG
  AGTTCAAAACTGCTATGAGTAATACTGCTAATCCTCAAAGCGTCACGAGGAAGGCTCAGAGACTCGAGGG
  CACAGCCCAACTCTGCGACTGCGCCAATTTCCTGCGAACGAGTTGCCCCCCCGTCCTGGGCTCTCAGGGC
  CTGGAGGTTCTTGCCGCACTGGTGTTTAAAAACCAGCGCTCCTTGAGGACACTGCAGGTTGAATTTCCGG
  CATTGGGACAGAAAACTCTCCCCACAAGCACAACCGGACTGCTCAACTTGCTCTCTCGGTGGCAGGACGG
  GGCTTTGCGCGCTCGACTTGACAGGCCAAGGCCCACAGCCCAGGGCCACCGCCCAAGGACTCACGTGGGT
  CCTAAGCCTTCACAGCTCACCGCAAGAGTACCTAGATCTGCACGGGCTGGTAGAGCTGGCGGTCGGAAGG
  GGCAGGTGGGTGCAGTAGGTCAGGTCTGCCCAGGTGCCCAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101075 representing YP_001129498
  Red=Cloning sites Green=Tags
  
  MDPTRGLCALSTHDLAKFHSLPPARKAAGKRAHLRCYSKLLSLKSWEQLASFLSLPPGPTFTDFRLFFEV
  TLGRRIADCVVVALQPSPRCYIVEFKTAMSNTANPQSVTRKAQRLEGTAQLCDCANFLRTSCPPVLGSQG
  LEVLAALVFKNQRSLRTLQVEFPALGQKTLPTSTTGLLNLLSRWQDGALRARLDRPRPTAQGHRPRTHVG
  PKPSQLTARVPRSARAGRAGGRKGQVGAVGQVCPGAQK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 744 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129498
• RefSeq ORF: 744 bp
• Locus ID: 5176174
• MW: 27.0 kDa