thymidine kinase (NC_009334) Virus Tagged ORF Clone

CAT#: VC101074

Myc-DDK-tagged ORF clone of viral ORF for BXLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129497

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: thymidine kinase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101074 represents NCBI reference of YP_001129497 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGATTTCCGGGAAAAGAAGCTGGCCCCCCGGGCGGCTGGAGAAAGTGCCAGGAAGACGAATCTC
  CCGAAAACGAGCGGCATGAAAACTTCTATGCTGAGATAGATGATTTCGCACCATCAGTTCTGACCCCAAC
  AGGGAGCGACTCTGGCGCTGGCGAAGAGGACGACGATGGCCTCTACCAAGTCCCTACACACTGGCCGCCG
  CTTATGGCTCCCACTGGCCTGAGTGGAGAACGGGTCCCTTGTAGAACTCAGGCCGCCGTAACGTCCAACA
  CTGGAAACTCTCCGGGTTCCAGGCATACCTCTTGCCCGTTCACCCTTCCGCGAGGAGCTCAACCCCCTGC
  ACCGGCTCATCAGAAGCCAACAGCACCAACCCCTAAACCCAGGTCTCGGGAATGCGGGCCAAGCAAGACA
  CCAGACCCATTCTCATGGTTCCGCAAAACCTCATGCACAGAAGGGGGGGCAGACAGCACCAGTCGCTCAT
  TTATGTACCAGAAAGGCTTTGAAGAGGGTTTGGCCGGACTGGGGCTCGACGATAAAAGCGATTGTGAATC
  CGAGGACGAGAGTAACTTTAGGAGACCAAGTAGCCACTCCGCTCTGAAGCAGAAGAACGGGGGTAAGGGA
  AAACCTTCTGGACTGTTCGAGCACCTGGCGGCACACGGCCGAGAGTTTAGCAAATTGTCAAAACACGCCG
  CCCAACTTAAGCGGCTTAGCGGATCTGTTATGAACGTTCTTAACCTGGATGATGCCCAAGATACCAGACA
  GGCTAAAGCTCAGCGAAAGGAGAGCACCAGGGTGCCCATTGTGACACACCTGACAAACCACGTCCCGGTG
  ATCAAACCCGCCTGCTCCCTTTTCCTGGAGGGGGCTCCTGGAGTTGGTAAGACCACCATGCTCAATCACC
  TTAAAGCCGTGTTCGGAGACCTTACCATCGTCGTCCCAGAGCCTATGCGCTATTGGACTCATGTGTATGA
  AAATGCCATAAAAGCAATGCATAAGAATGTTACCCGGGCAAGGCACGGGAGAGAGGATACTTCCGCTGAG
  GTCCTCGCCTGTCAGATGAAGTTTACCACCCCATTCAGAGTCCTGGCTAGTCGGAAAAGATCACTGCTGG
  TCACAGAGTCAGGTGCACGCAGCGTGGCCCCTCTGGACTGCTGGATCCTCCACGACCGACACCTCCTCTC
  AGCCTCTGTCGTGTTTCCTCTTATGCTGCTGAGAAGTCAGTTGCTTTCCTATAGTGATTTCATCCAGGTA
  CTCGCCACATTCACAGCGGATCCGGGCGATACCATTGTCTGGATGAAGTTGAATGTGGAAGAGAACATGA
  GGCGGCTGAAGAAGCGGGGCAGGAAGCATGAGAGCGGTTTGGATGCCGGCTACCTGAAATCAGTGAACGA
  TGCCTATCACGCAGTGTATTGTGCTTGGCTCCTTACCCAGTACTTTGCGCCTGAGGACATCGTTAAGGTG
  TGTGCTGGGCTTACAACCATTACAACCGTGTGCCATCAATCCCATACACCGATCATCCGCTCAGGGGTTG
  CCGAGAAGCTCTACAAGAATTCTATTTTCAGTGTGCTTAAGGAAGTGATACAGCCATTCAGGGCAGATGC
  AGTTCTTTTGGAAGTTTGCCTCGCTTTTACCAGGACCCTGGCTTATCTGCAGTTTGTACTCGTTGACCTC
  TCCGAGTTTCAGGACGATCTCCCTGGGTGCTGGACTGAAATATATATGCAGGCCCTCAAGAATCCTGCCA
  TTAGATCTCAATTCTTCGACTGGGCTGGACTCTCCAAGGTTATTAGTGATTTCGAGAGAGGTAATCGGGA
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101074 representing YP_001129497
  Red=Cloning sites Green=Tags
  
  MAGFPGKEAGPPGGWRKCQEDESPENERHENFYAEIDDFAPSVLTPTGSDSGAGEEDDDGLYQVPTHWPP
  LMAPTGLSGERVPCRTQAAVTSNTGNSPGSRHTSCPFTLPRGAQPPAPAHQKPTAPTPKPRSRECGPSKT
  PDPFSWFRKTSCTEGGADSTSRSFMYQKGFEEGLAGLGLDDKSDCESEDESNFRRPSSHSALKQKNGGKG
  KPSGLFEHLAAHGREFSKLSKHAAQLKRLSGSVMNVLNLDDAQDTRQAKAQRKESTRVPIVTHLTNHVPV
  IKPACSLFLEGAPGVGKTTMLNHLKAVFGDLTIVVPEPMRYWTHVYENAIKAMHKNVTRARHGREDTSAE
  VLACQMKFTTPFRVLASRKRSLLVTESGARSVAPLDCWILHDRHLLSASVVFPLMLLRSQLLSYSDFIQV
  LATFTADPGDTIVWMKLNVEENMRRLKKRGRKHESGLDAGYLKSVNDAYHAVYCAWLLTQYFAPEDIVKV
  CAGLTTITTVCHQSHTPIIRSGVAEKLYKNSIFSVLKEVIQPFRADAVLLEVCLAFTRTLAYLQFVLVDL
  SEFQDDLPGCWTEIYMQALKNPAIRSQFFDWAGLSKVISDFERGNRD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1821 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129497
• RefSeq ORF: 1821 bp
• Locus ID: 5176155
• MW: 67.2 kDa