BFLF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101021

Myc-DDK-tagged ORF clone of viral ORF for BFLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129444

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BFLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101021 represents NCBI reference of YP_001129444 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCATAAAGTGACAAGCGCGAACGAGCCCAATCCTCTTACGGGTAAGAGACTCAGTTCTTGTCCAC
  TTACTCGAAGTGGCGTGACCGAAGTTGCCCAAATCGCCGGTCGGACTCCAAAAATGGAAGACTTCGTGCC
  ATGGACGGTGGATAATCTCAAAAGCCAGTTTGAGGCTGTCGGTCTGCTTATGGCCCACTCCTATTTGCCA
  GCCAACGCGGAAGAAGGTATAGCTTACCCCCCCCTTGTGCACACCTACGAATCATTGAGCCCCGCCAGCA
  CATGCAGAGTGTGCGACCTCTTGGACACGCTGGTCAACCACTCTGACGCGCCTGTGGCATTCTTCGAGGA
  TTATGCCCTCCTGTGCTACTATTGTCTCAATGCACCTCGAGCATGGATTAGCTCTCTCATCACTGGTATG
  GACTTTCTGCATATCCTCATAAAATACTTCCCAATGGCCGGGGGGCTGGACAGTCTGTTCATGCCCTCCC
  GGATTCTGGCAATAGATATTCAGCTTCACTTCTATATTTGTAGATGCTTTCTTCCAGTGTCTTCCTCAGA
  CATGATTAGAAATGCTAATCTTGGTTACTACAAGCTGGAGTTCCTGAAGTCCATCCTGACGGGACAAAGT
  CCTGCCAACTTCTGTTTCAAATCTATGTGGCCACGCACTACCCCTACATTTCTGACCCTGCCTGGCCCAC
  GCACTTGTAAAGATTCTCAAGATGTGCCAGGAGATGTCGGACGCGGTCTCTACACTGCTCTGTGCTGCCA
  CCTCCCTACTCGAAACCGGGTTCAGCATCCTTTTCTCAGAGCCGAAAAGGGCGGTTTGAGCCCTAAGATC
  ACTACAAAAGCTGATTATTGTGGTCTGCTGCTGGGAACTTGGCAAGGCACTGACCTGCTTGGGGGACCAG
  GGCATCATGCCATCGGTCTGAACGCCGAGTATTCAGGCGACGAGTTGGCTGAACTGGCCCTTGCCATAAC
  CAGACCTGAGGCCGGGGATCACTCCCAGGGACCATGTCTGCTGGCACCCATGTTTGGCCTCCGACATAAG
  AACGCCTCCCGCACCATATGTCCGCTCTGCGAGAGCCTGGGGGCACATCCGGACGCAAAAGATACGTTGG
  ATCGATTTAAGTCCCTCATTCTGGATTCTTTCGGGAATAATATTAAGATCCTGGACCGCATAGTCTTTTT
  GATTAAAACTCAGAACACCCTGTTGGACGTACCTTGCCCCAGACTGCGGGCTTGGCTTCAGATGTGCACC
  CCGCAGGATTTTCACAAACACTTGTTTTGTGATCCACTGTGCGCAATTAATCATAGTATCACAAACCCAT
  CTGTGCTCTTCGGGCAGATTTACCCCCCTTCCTTTCAGGCATTTAAGGCTGCACTGGCCGCCGGCCAGAA
  CCTGGAGCAGGGCGTCTGCGACTCACTCATCACCCTGGTTTATATCTTTAAAAGCACTCAAGTCGCTAGA
  GTAGGAAAAACAATCCTGGTGGATGTGACTAAAGAATTGGACGTTGTACTGCGGATTCACGGCCTGGACC
  TTGTGCAGTCTTATCAGACCTCCCAGGTGTATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101021 representing YP_001129444
  Red=Cloning sites Green=Tags
  
  MAHKVTSANEPNPLTGKRLSSCPLTRSGVTEVAQIAGRTPKMEDFVPWTVDNLKSQFEAVGLLMAHSYLP
  ANAEEGIAYPPLVHTYESLSPASTCRVCDLLDTLVNHSDAPVAFFEDYALLCYYCLNAPRAWISSLITGM
  DFLHILIKYFPMAGGLDSLFMPSRILAIDIQLHFYICRCFLPVSSSDMIRNANLGYYKLEFLKSILTGQS
  PANFCFKSMWPRTTPTFLTLPGPRTCKDSQDVPGDVGRGLYTALCCHLPTRNRVQHPFLRAEKGGLSPKI
  TTKADYCGLLLGTWQGTDLLGGPGHHAIGLNAEYSGDELAELALAITRPEAGDHSQGPCLLAPMFGLRHK
  NASRTICPLCESLGAHPDAKDTLDRFKSLILDSFGNNIKILDRIVFLIKTQNTLLDVPCPRLRAWLQMCT
  PQDFHKHLFCDPLCAINHSITNPSVLFGQIYPPSFQAFKAALAAGQNLEQGVCDSLITLVYIFKSTQVAR
  VGKTILVDVTKELDVVLRIHGLDLVQSYQTSQVYV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129444
• RefSeq ORF: 1575 bp
• Locus ID: 5176154
• MW: 57.9 kDa