BALF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101087

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BALF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129511


  View other clones from "Virus" (71)

Reconstitution Protocol

USD 330.00

3 Weeks*

Size
    • 10 ug

Product Images

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol BALF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101087 represents NCBI reference of YP_001129511 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGACCTGCGAAATCTACAGACAGTGTATTTGTTCGCACCCCCGTTGAGGCTTGGGTCGCACCTAGTC
CACCTGACGACAAGGTTGCTGAGTCCTCCTACCTGATGTTTAGAGCAATGTATGCCGTGTTCACTAGAGA
TGAGAAAGACCTCCCTCTGCCTGCTTTGGTCCTTTGTCGCCTGATTAAAGCTAGTCTTAGAAAAGATCGA
AAGCTTTATGCCGAACTGGCTTGTCGCACAGCGGATATTGGCGGGAAAGATACCCATGTGCGGCTGATCA
TCTCTGTTCTGCGAGCCGTTTATAACGACCACTACGATTACTGGTCACGGCTGCGGGTGGTGCTTTGTTA
TACTGTCGTTTTTGCCGTGCGGAATTACCTGGACGACCATAAGAGCGCCGCGTTCGTGCTGGGCGCAATT
GCCCACTATCTGGCCCTCTACAGGCGGCTGTGGTTTGCCCGGCTTGGCGGCATGCCAAGATCCCTGAGAC
GCCAGTTCCCCGTGACTTGGGCTCTGGCAAGTCTCACTGACTTCCTTAAGAGCCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101087 representing YP_001129511
Red=Cloning sites Green=Tags

MRPAKSTDSVFVRTPVEAWVAPSPPDDKVAESSYLMFRAMYAVFTRDEKDLPLPALVLCRLIKASLRKDR
KLYAELACRTADIGGKDTHVRLIISVLRAVYNDHYDYWSRLRVVLCYTVVFAVRNYLDDHKSAAFVLGAI
AHYLALYRRLWFARLGGMPRSLRRQFPVTWALASLTDFLKSL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_009334
ORF Size 546 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NC_009334.1, YP_001129511
RefSeq ORF 546 bp
Locus ID 5176161
MW 20.9 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.