BVRF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101077

Myc-DDK-tagged ORF clone of viral ORF for BVRF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129501

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BVRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101077 represents NCBI reference of YP_001129501 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGGCTCCATCTGTGTACGTGTGCGGTTTCGTGGAGAGACCTGACGCACCCCCTAAAGACGCAT
  GCCTTCATCTCGATCCCCTTACAGTCAAAAGTCAACTTCCACTTAAAAAGCCCCTGCCTCTTACAGTGGA
  ACATCTGCCAGACGCTCCAGTCGGTAGTGTGTTTGGGCTCTATCAGAGCAGAGCTGGTCTGTTCAGCGCT
  GCTAGTATTACTTCTGGAGTTTTCCTGTCCCTGTTGGACAGCATATACCACGACTGCGACATTGCCCAGA
  GTCAGAGACTCCCACTGCCTCGGGAACCAAAGCTGGAAGCACTTCACGCATGGCTCCCCTCTCTTAGCCT
  GGCCAGCCTGCATCCCGATATACCTCAAACAACAGCAGATGGGGGCAAGCTGTCATTCTTCGATCACGTT
  TCCATTTGTGCACTGGGGAGAAGACGGGGGACGACAGCTGTGTACGGGACCGACTTGGCCTGGGTACTGA
  AGCACTTTTCTGACCTGGAGCCAAGTATCGCCGCGCAGATCGAAAATGATGCAAATGCCGCCAAGCGCGA
  AAGTGGTTGTCCAGAGGATCATCCGCTGCCACTCACCAAGCTGATCGCCAAAGCCATCGATGCCGGCTTC
  CTGAGAAATCGCGTGGAGACCCTGCGCCAAGACAGGGGAGTGGCCAACATCCCCGCCGAATCTTACTTGA
  AAGCATCTGACGCCCCCGATCTGCAGAAGCCTGATAAGGCCCTGCAATCACCCCCCCCTGCCTCAACCGA
  CCCTGATACAATGTTGTCCGGGAATGCTGGGGAGGGGGCTACGGCTTGCGGAGGGAGCGCGGCCGCCGGA
  CAAGACCTGATAAGCGTGCCAAGAAACACATTCATGACGCTGCTCCAGACTAATCTGGACAACAAGCCAC
  CGCGCCAGACACCCCTGCCCTATGCCGCTCCTCTCCCTCCATTTAGCCACCAAGCTATCGCCACCGCACC
  CTCTTACGGACCAGGTGCTGGAGCGGTGGCTCCCGCGGGAGGGTATTTCACCTCACCTGGAGGGTACTAT
  GCTGGCCCAGCTGGCGGCGATCCAGGGGCCTTCTTGGCCATGGATGCACACACTTACCACCCCCACCCAC
  ACCCTCCTCCTGCCTACTTTGGGCTCCCCGGCCTTTTTGGCCCGCCCCCACCAGTGCCACCCTACTACGG
  CTCCCACCTGAGAGCTGACTACGTCCCCGCCCCGTCTCGCTCAAACAAGAGGAAGAGAGACCCCGAGGAG
  GACGAGGAGGGGGGTGGCCTCTTTCCGGGAGAGGACGCCACCCTTTACAGGAAGGACATTGCTGGGCTGA
  GCAAGTCAGTAAATGAGCTGCAGCACACTCTGCAAGCCCTGCGCAGGGAGACCCTTAGTTATGGGCATAC
  AGGAGTGGGCTACTGTCCGCAGCAGGGGCCTTGCTATACACACCCCGGACCTTACGGCTTTCAGCCTCAT
  CAATCCTACGAGGTGCCGCGCTACGTCCCACATCCGCCCCCTCCTCCAACGAGCCATCAGGCCGCGCAGG
  CTCAACCGCCTCCTCCTGGCACTCAGGCACCAGAAGCCCATTGTGTGGCTGAGAGCACCATTCCCGAGGC
  TGGGGCCGCAGGCAATAGTGGCCCCCGGGAAGACACGAATCCACAGCAGCCTACCACCGAGGGACACCAT
  CGGGGAAAAAAGCTCGTCCAGGCTTCAGCCAGCGGGGTAGCCCAATCCAAAGAACCCACGACCCCAAAGG
  CTAAATCTGTGAGTGCGCACCTTAAGTCCATCTTTTGCGAAGAGCTTCTGAACAAACGAGTGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101077 representing YP_001129501
  Red=Cloning sites Green=Tags
  
  MVQAPSVYVCGFVERPDAPPKDACLHLDPLTVKSQLPLKKPLPLTVEHLPDAPVGSVFGLYQSRAGLFSA
  ASITSGVFLSLLDSIYHDCDIAQSQRLPLPREPKLEALHAWLPSLSLASLHPDIPQTTADGGKLSFFDHV
  SICALGRRRGTTAVYGTDLAWVLKHFSDLEPSIAAQIENDANAAKRESGCPEDHPLPLTKLIAKAIDAGF
  LRNRVETLRQDRGVANIPAESYLKASDAPDLQKPDKALQSPPPASTDPDTMLSGNAGEGATACGGSAAAG
  QDLISVPRNTFMTLLQTNLDNKPPRQTPLPYAAPLPPFSHQAIATAPSYGPGAGAVAPAGGYFTSPGGYY
  AGPAGGDPGAFLAMDAHTYHPHPHPPPAYFGLPGLFGPPPPVPPYYGSHLRADYVPAPSRSNKRKRDPEE
  DEEGGGLFPGEDATLYRKDIAGLSKSVNELQHTLQALRRETLSYGHTGVGYCPQQGPCYTHPGPYGFQPH
  QSYEVPRYVPHPPPPPTSHQAAQAQPPPPGTQAPEAHCVAESTIPEAGAAGNSGPREDTNPQQPTTEGHH
  RGKKLVQASASGVAQSKEPTTPKAKSVSAHLKSIFCEELLNKRVA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129501
• RefSeq ORF: 1815 bp
• Locus ID: 5176187
• MW: 64.2 kDa