BILF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101079

Myc-DDK-tagged ORF clone of viral ORF for BILF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129503

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BILF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101079 represents NCBI reference of YP_001129503 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCATTTGGTGCTGCTGCTGTGCTGTTGTGTGGGTTCCGTGTGCGCGTTCTTCAGCGACCTCGTTA
  AGTTCGAAAATGTGACAGCGCACGCCGGCGCCAGAGTGAACCTTACATGCTCCGTCCCGTCCAATGAGTC
  CGTGTCCAGGATCGAGTTGGGAAGGGGATACACCCCAGGCGATGGTCAACTGCCATTGGCAGTCGCCACC
  TCTAACAACGGTACACATATTACCAACGGTGGCTACAACTATTCCCTGACGCTCGAATGGGTGAACGATA
  GCAATACATCTGTGAGCCTGATCATTCCAAACGTGACACTGGCCCATGCAGGCTATTATACGTGCAACGT
  GACGTTGCGAAACTGCTCCGTGGCATCTGGAGTGCATTGCAACTACTCTGCAGGGGAGGAGGATGATCAA
  TATCACGCCAATCGAACCCTGACCCAGCGAATGCACCTGACCGTGATACCTGCTACAACGATCGCCCCTA
  CTACCCTCGTCTCCCATACCACTAGCACCAGCCACCGCCCCCATCGAAGACCCGTCTCAAAGCGGCCCAC
  ACACAAGCCCGTTACTCTCGGACCTTTTCCAATAGATCCATGGAGACCTAAAACAACATGGGTGCATTGG
  GCTCTGCTGCTCATTACCTGTGCGGTGGTCGCTCCAGTTTTGCTGATAATCATTATTTCATGTCTCGGTT
  GGTTGGCCGGCTGGGGTCGAAGACGCAAGGGTTGGATACCACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101079 representing YP_001129503
  Red=Cloning sites Green=Tags
  
  MTHLVLLLCCCVGSVCAFFSDLVKFENVTAHAGARVNLTCSVPSNESVSRIELGRGYTPGDGQLPLAVAT
  SNNGTHITNGGYNYSLTLEWVNDSNTSVSLIIPNVTLAHAGYYTCNVTLRNCSVASGVHCNYSAGEEDDQ
  YHANRTLTQRMHLTVIPATTIAPTTLVSHTTSTSHRPHRRPVSKRPTHKPVTLGPFPIDPWRPKTTWVHW
  ALLLITCAVVAPVLLIIIISCLGWLAGWGRRRKGWIPL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 744 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129503
• RefSeq ORF: 744 bp
• Locus ID: 5176166
• MW: 27.1 kDa