glycoprotein BALF4 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101084

Myc-DDK-tagged ORF clone of viral ORF for BALF4 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129508

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: glycoprotein BALF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101084 represents NCBI reference of YP_001129508 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACGGCGCCGGGTGTTGTCTGTCGTAGTCCTGCTTGCCGCTCTGGCCTGTCGCCTCGGCGCCCAGA
  CACCTGAACAACCAGCACCACCGGCCACCACAGTCCAGCCCACAGCCACCAGACAACAGACGTCCTTCCC
  ATTCAGAGTGTGTGAACTTTCCAGTCACGGCGACCTGTTTAGGTTTAGCAGCGATATCCAGTGCCCCTCT
  TTCGGAACCAGGGAAAACCACACCGAGGGGCTCCTGATGGTGTTTAAGGACAATATTATCCCATATAGCT
  TCAAAGTTCGGTCATACACAAAGATTGTGACCAACATCCTGATCTACAATGGCTGGTATGCAGATTCCGT
  AACCAACAGACATGAGGAGAAATTCTCCGTGGATAGTTATGAGACCGACCAGATGGATACCATTTACCAG
  TGCTACAACGCAGTTAAGATGACAAAGGATGGCCTTACCCGCGTGTACGTGGATCGAGACGGGGTGAATA
  TAACAGTAAATCTCAAACCAACTGGGGGTCTGGCAAATGGCGTGAGGCGCTACGCCTCTCAGACTGAATT
  GTACGATGCTCCCGGCTGGCTTATCTGGACCTACAGAACCCGGACCACAGTAAACTGCTTGATTACTGAC
  ATGATGGCGAAGAGTAACAGTCCATTTGACTTTTTTGTAACGACTACAGGCCAGACGGTGGAGATGTCTC
  CTTTCTATGATGGTAAAAATAAGGAGACGTTTCATGAGCGCGCAGATTCATTTCACGTCCGGACCAACTA
  TAAAATAGTGGACTACGATAACAGGGGGACCAATCCCCAGGGGGAGCGACGCGCCTTTCTGGATAAAGGC
  ACATATACTCTCTCATGGAAACTGGAAAACCGGACTGCATATTGTCCTCTGCAGCACTGGCAGACTTTTG
  ATAGTACAATCGCCACCGAAACGGGGAAATCCATCCACTTTGTGACGGATGAAGGCACTTCTTCCTTCGT
  GACTAACACCACAGTCGGCATTGAGCTGCCCGATGCATTCAAGTGTATCGAAGAGCAGGTGAACAAAACG
  ATGCATGAGAAGTACGAAGCAGTGCAGGACAGATACACCAAGGGCCAGGAGGCCATTACGTACTTCATCA
  CTTCCGGCGGTCTGCTCCTTGCCTGGCTGCCGCTCACTCCTCGAAGTCTGGCCACCGTCAAGAACCTCAC
  TGAGCTGACAACCCCCACATCTTCCCCTCCGTCTTCTCCATCCCCACCCGCCCCTCCAGCGGCGAGAGGA
  TCTACCAGCGCAGCCGTGCTGAGAAGACGAAGACGCGACGCAGGCAACGCGACAACTCCTGTTCCTCCCG
  CCGCACCCGGTAAATCTCTGGGAACCCTGAACAACCCAGCTACTGTGCAAATTCAGTTCGCATATGATAG
  TCTGAGAAGGCAGATCAATAGAATGCTGGGCGATCTCGCCCGAGCCTGGTGCTTGGAGCAGAAGCGGCAA
  AATATGGTCTTGAGAGAGCTGACAAAGATCAACCCCACAACTGTTATGTCAAGCATTTACGGTAAGGCTG
  TTGCAGCTAAGCGACTCGGTGATGTGATTAGCGTGAGTCAGTGTGTGCCAGTCAATCAGGCAACTGTGAC
  ACTTCGCAAGTCAATGCGGGTCCCCGGGAGTGAGACAATGTGTTATTCTCGGCCTCTGGTTTCATTCAGT
  TTTATCAACGACACTAAGACCTATGAAGGGCAACTTGGAACAGACAATGAGATTTTCCTGACCAAGAAGA
  TGACTGAGGTGTGTCAGGCCACTAGCCAGTACTATTTCCAGAGCGGGAACGAAATTCACGTGTATAATGA
  TTACCACCACTTTAAGACCATTGAACTGGACGGCATAGCCACACTCCAGACTTTCATTTCCCTGAATACC
  AGTCTGATAGAAAATATTGACTTTGCCAGCCTGGAACTGTACAGCCGCGACGAGCAGAGAGCCTCCAATG
  TGTTTGACCTGGAAGGGATTTTTCGAGAGTACAATTTCCAGGCACAGAACATAGCTGGGCTGAGAAAGGA
  CCTGGACAATGCTGTTAGCAATGGTCGGAATCAATTTGTGGACGGCCTGGGGGAACTGATGGATAGTCTG
  GGCAGCGTCGGTCAGAGCATAACCAACCTGGTGTCCACTGTTGGGGGACTCTTTTCCTCTCTCGTGAGTG
  GCTTTATTTCATTTTTTAAGAACCCATTTGGGGGAATGCTGATCCTGGTCTTGGTGGCAGGCGTCGTGAT
  CCTGGTAATCAGCCTCACCAGGAGAACTAGACAGATGTCCCAGCAGCCGGTACAGATGTTGTATCCCGGC
  ATCGACGAGTTGGCTCAACAGCACGCCAGCGGGGAAGGTCCAGGAATCAATCCTATAAGCAAAACCGAGC
  TCCAGGCCATTATGCTGGCCCTGCACGAACAAAATCAAGAACAGAAAAGAGCGGCCCAGCGCGCAGCCGG
  GCCAAGCGTCGCCAGTAGAGCCCTTCAGGCAGCTAGAGATCGGTTCCCCGGACTCCGAAGACGCAGGTAT
  CATGACCCCGAAACAGCAGCCGCGCTTCTCGGTGAGGCCGAGACCGAATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101084 representing YP_001129508
  Red=Cloning sites Green=Tags
  
  MTRRRVLSVVVLLAALACRLGAQTPEQPAPPATTVQPTATRQQTSFPFRVCELSSHGDLFRFSSDIQCPS
  FGTRENHTEGLLMVFKDNIIPYSFKVRSYTKIVTNILIYNGWYADSVTNRHEEKFSVDSYETDQMDTIYQ
  CYNAVKMTKDGLTRVYVDRDGVNITVNLKPTGGLANGVRRYASQTELYDAPGWLIWTYRTRTTVNCLITD
  MMAKSNSPFDFFVTTTGQTVEMSPFYDGKNKETFHERADSFHVRTNYKIVDYDNRGTNPQGERRAFLDKG
  TYTLSWKLENRTAYCPLQHWQTFDSTIATETGKSIHFVTDEGTSSFVTNTTVGIELPDAFKCIEEQVNKT
  MHEKYEAVQDRYTKGQEAITYFITSGGLLLAWLPLTPRSLATVKNLTELTTPTSSPPSSPSPPAPPAARG
  STSAAVLRRRRRDAGNATTPVPPAAPGKSLGTLNNPATVQIQFAYDSLRRQINRMLGDLARAWCLEQKRQ
  NMVLRELTKINPTTVMSSIYGKAVAAKRLGDVISVSQCVPVNQATVTLRKSMRVPGSETMCYSRPLVSFS
  FINDTKTYEGQLGTDNEIFLTKKMTEVCQATSQYYFQSGNEIHVYNDYHHFKTIELDGIATLQTFISLNT
  SLIENIDFASLELYSRDEQRASNVFDLEGIFREYNFQAQNIAGLRKDLDNAVSNGRNQFVDGLGELMDSL
  GSVGQSITNLVSTVGGLFSSLVSGFISFFKNPFGGMLILVLVAGVVILVISLTRRTRQMSQQPVQMLYPG
  IDELAQQHASGEGPGINPISKTELQAIMLALHEQNQEQKRAAQRAAGPSVASRALQAARDRFPGLRRRRY
  HDPETAAALLGEAETEF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 2571 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129508
• RefSeq ORF: 2571 bp
• Locus ID: 5176207
• MW: 95.6 kDa