BBRF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101053

Myc-DDK-tagged ORF clone of viral ORF for BBRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129476

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BBRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101053 represents NCBI reference of YP_001129476 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAACATGAACGTGGATGAAAGTGCCTCTGGCGCCCTCGGGTCCAGCGCAATTCCCGTGCATCCTA
  CCCCAGCTTCCGTAAGACTGTTCGAGATTCTTCAGGGAAAATATGCGTACGTGCAAGGGCAGACTATATA
  TGCTAACCTGAGGAACCCAGGGGTGTTCAGCAGACAGGTATTTACCCACCTGTTTAAGAGAGCTATCTCC
  CATTGCACATATGATGACGTGCTGCACGATTGGAACAAGTTCGAGGCTTGCATTCAGAAGAGGTGGCCCT
  CAGACGATAGTTGTGCATCACGCTTTAGGGAATCCACCTTTGAGTCCTGGTCCACCACGATGAAGCTCAC
  AGTCAGGGACCTCCTTACGACGAATATCTACCGAGTCCTTCACTCTCGGTCTGTGTTGAGTTATGAACGA
  TACGTCGACTGGATATGCGCCACGGGCATGGTTCCAGCAGTCAAGAAACCAATTACCCAAGAACTGCATT
  CCAAGATCAAAAGCCTTCGGGACAGGTGCGTTTGTAGGGAGCTGGGCCATGAGAGGACCATACGCAGCAT
  TGGGACTGAACTGTATGAGGCCACTCGGGAGATTATCGAGTCCCTGAATTCTACCTTTATCCCCCAATTC
  ACCGAGGTAACCATTGAGTACCTCCCTGGCTCCGACGAATACGTTGCATACTATTGCGGAAGACGCATTC
  GGCTGCACGTGCTTTTTCCTCCTGCCATTTTCGCAGGGACCGTTACCTTCGACAGTCCTGTACAACGCCT
  GTACCAAAACATATTTATGTGCTATCGAACCCTCGAACATGCCAAGATCTGCCAGCTGCTCAATACCGCC
  CCCCTGAAAGCAATTGTAGGCCACGGAGGAAGGGACATGTACAAGGACATTCTCGCGCACCTCGAGCAGA
  ATTCACAAAGAAAGGATCCTAAGAAAGAACTTTTGAATCTTCTGGTGAAACTGTCTGAGAACAAGACCAT
  CAGCGGAGTAACGGACGTAGTGGAGGAATTTATCACCGACGCCTCAAACAATTTGGTGGATAGGAATCGG
  TTGTTCGGTCAGCCCGGCGAGACGGCAGCCCAAGGTCTCAAGAAAAAGGTCAGCAATACGGTGGTCAAAT
  GTCTCACTGATCAAATCAACGAGCAATTCGACCAGATTAATGGATTGGAAAAGGAAAGAGAACTCTACCT
  TAAAAAGATTAGATCCATGGAGAGTCAGCTGCAAGCTAGCCTCGGCCCGGGCGGTAACAACCCAGCGGCA
  AGCGCACCTGCTGCTGTAGCTGCAGAGGCCGCTTCCGTGGATATTCTTACCGGATCTACCGCATCCGCAA
  TCGAAAAGCTCTTTAATTCCCCTTCAGCCTCTCTGGGAGCTCGGGTGTCTGGCCACAACGAGTCTATCCT
  GAACAGCTTCGTGTCTCAATATATCCCCCCATCAAGGGAGATGACTAAAGATCTCACCGAACTGTGGGAA
  TCCGAGTTGTTCAATACCTTTAAGTTGACTCCCGTGGTCGATAATCAAGGACAAAGATTGTACGTACGCT
  ATAGTTCCGATACAATAAGCATCCTGCTGGGACCCTTTACGTATCTCGTGGCGGAACTTAGCCCCGTAGA
  GTTGGTTACTGATGTATATGCCACCCTTGGTATAGTGGAAATCATAGACGAACTGTATCGCTCATCAAGG
  CTGGCAATCTACATCGAAGATCTGGGACGCAAATATTGCCCAGCTTCCGCTACAGGAGGCGACCACGGCA
  TAAGGCAAGCCCCCTCAGCACGAGGCGACGCTGAGCCAGACCATGCCAAGAGCAAACCTGCCCGCGATCC
  CCCACCAGGCGCTGGATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101053 representing YP_001129476
  Red=Cloning sites Green=Tags
  
  MFNMNVDESASGALGSSAIPVHPTPASVRLFEILQGKYAYVQGQTIYANLRNPGVFSRQVFTHLFKRAIS
  HCTYDDVLHDWNKFEACIQKRWPSDDSCASRFRESTFESWSTTMKLTVRDLLTTNIYRVLHSRSVLSYER
  YVDWICATGMVPAVKKPITQELHSKIKSLRDRCVCRELGHERTIRSIGTELYEATREIIESLNSTFIPQF
  TEVTIEYLPGSDEYVAYYCGRRIRLHVLFPPAIFAGTVTFDSPVQRLYQNIFMCYRTLEHAKICQLLNTA
  PLKAIVGHGGRDMYKDILAHLEQNSQRKDPKKELLNLLVKLSENKTISGVTDVVEEFITDASNNLVDRNR
  LFGQPGETAAQGLKKKVSNTVVKCLTDQINEQFDQINGLEKERELYLKKIRSMESQLQASLGPGGNNPAA
  SAPAAVAAEAASVDILTGSTASAIEKLFNSPSASLGARVSGHNESILNSFVSQYIPPSREMTKDLTELWE
  SELFNTFKLTPVVDNQGQRLYVRYSSDTISILLGPFTYLVAELSPVELVTDVYATLGIVEIIDELYRSSR
  LAIYIEDLGRKYCPASATGGDHGIRQAPSARGDAEPDHAKSKPARDPPPGAGS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1839 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129476
• RefSeq ORF: 1839 bp
• Locus ID: 5176175
• MW: 68.4 kDa