BdRF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101078

Myc-DDK-tagged ORF clone of viral ORF for BdRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129502

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BdRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101078 represents NCBI reference of YP_001129502 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAGCGGCAATGCTGGAGAGGGCGCAACAGCTTGCGGCGGCTCTGCCGCAGCTGGGCAGGATCTTA
  TCTCCGTGCCCAGGAATACCTTCATGACGCTGCTGCAAACAAACCTCGACAACAAGCCACCACGACAGAC
  TCCACTGCCATACGCCGCACCCCTCCCACCTTTCTCACACCAGGCGATTGCAACCGCACCGTCTTACGGC
  CCTGGGGCTGGAGCCGTTGCACCCGCTGGGGGGTATTTTACTTCCCCGGGCGGGTATTATGCAGGCCCTG
  CCGGGGGTGACCCCGGCGCATTTCTGGCCATGGACGCCCACACCTACCACCCCCATCCACACCCTCCTCC
  AGCTTATTTTGGTCTCCCAGGGCTGTTTGGCCCACCACCCCCAGTCCCACCTTATTATGGTTCTCACCTG
  CGGGCTGATTATGTCCCAGCTCCGTCACGGTCTAACAAGAGGAAGCGGGATCCTGAAGAGGATGAGGAGG
  GTGGGGGCCTTTTTCCCGGGGAAGATGCCACACTCTACAGGAAAGATATCGCTGGCCTGTCCAAAAGCGT
  GAACGAGCTGCAGCATACCTTGCAGGCATTGCGCAGAGAGACTCTCAGCTATGGCCACACCGGTGTCGGA
  TACTGTCCGCAGCAAGGCCCATGTTACACTCACCCGGGACCCTACGGCTTCCAGCCGCACCAGTCTTATG
  AAGTCCCCAGGTACGTCCCACACCCTCCACCCCCACCAACGTCCCATCAGGCCGCTCAGGCTCAGCCTCC
  GCCTCCAGGTACTCAAGCCCCCGAAGCACACTGCGTAGCAGAGTCAACCATCCCTGAAGCTGGCGCTGCA
  GGGAACAGTGGCCCCAGGGAAGACACAAACCCACAGCAACCAACTACAGAGGGCCATCACCGAGGAAAAA
  AACTGGTCCAGGCCTCAGCTAGTGGTGTGGCCCAGAGTAAGGAGCCGACTACCCCCAAGGCTAAATCCGT
  GAGCGCACATCTTAAATCAATATTCTGCGAGGAGCTCTTGAATAAAAGGGTAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101078 representing YP_001129502
  Red=Cloning sites Green=Tags
  
  MLSGNAGEGATACGGSAAAGQDLISVPRNTFMTLLQTNLDNKPPRQTPLPYAAPLPPFSHQAIATAPSYG
  PGAGAVAPAGGYFTSPGGYYAGPAGGDPGAFLAMDAHTYHPHPHPPPAYFGLPGLFGPPPPVPPYYGSHL
  RADYVPAPSRSNKRKRDPEEDEEGGGLFPGEDATLYRKDIAGLSKSVNELQHTLQALRRETLSYGHTGVG
  YCPQQGPCYTHPGPYGFQPHQSYEVPRYVPHPPPPPTSHQAAQAQPPPPGTQAPEAHCVAESTIPEAGAA
  GNSGPREDTNPQQPTTEGHHRGKKLVQASASGVAQSKEPTTPKAKSVSAHLKSIFCEELLNKRVA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1035 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129502
• RefSeq ORF: 1035 bp
• Locus ID: 5176196
• MW: 36.1 kDa