BRRF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101047

Myc-DDK-tagged ORF clone of viral ORF for BRRF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129470

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101047 represents NCBI reference of YP_001129470 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGCCAACAGCGCGGCTCCGTCATCCTGGTGCCGGAGCATTTGGCCGGTGCACTGACCAAACTTA
  TGAGCGATTTTATAACGGGCCAGGATGTCACACTGTCAGGAGGCAATATCGCAGTTAAAATACGGGATGC
  CATCAACCAAACACCAGGGGGAGGGGATGTTGCTATCCTGAGCTCACTTTTCGCCCTTTGGAATGCTCTG
  CCGACTAGCGGAAGGCAGTCTTCAAGAGATGACCTCATTCCTGCAGCAGTACAGGCACTGACTACTGCAC
  ACAACCTTTGCCTGGGCGTGATCCCCGGTGAGACAAGCCACAAGGACACACCCGAGTCTCTGCTTAGAGC
  TATCGTGACAGGCCTCCAGAAATTGTGGGTAGATAGCTGCGGGTGCCCTGAGTGCCTGCAGTGCTTGAAA
  GGATTGAAGGCGATCAAACCAGGCCTCTATGAGATCCCACGAATTATCCCCCACACCAAGCAGTGCAGCC
  CAGTCAACCTGCTGAATATGCTCGTCCACAAGCTGGTGGCCTTGAGAGGTCACGTTCAACTCGCGTACGA
  TGCACGGGTACTCACCCCAGATTTCCATGAAATACCTGACCTGGACGATTCAGACGCAGTCTTCGCCCGG
  ACACTCCTGGCTGCACTGTTCCACCTCAATATGTTCTTTATCCTTAAAGACTACATCACCCAGGATTCAA
  TGAGTCTGAAACAGGCCCTGTCAGGTCATTGGATGTCCGCTACTGGTAACCCCCTTCCCGCTGCCCCCGA
  AACACTTCGCGATTACTTGGAGGCCTTTAGGAATAGTGACAACCACTTTTACCTTCCTACCACCGGACCC
  CTCAACACTTTTAAATTCCCTGAGGAGCTTCTCGGGCGGGTAGTAGTGATCGATTCCTCCTTGTGTGCCG
  CTAGCCACGTTCAGGACGTCATTACTAGGGGCGTTGGAGCCGGTGTGCCTCGCCCCCAATTCTTGGCCCT
  GCCGCCTGCACCAAGCCGAAAACCTCAGCAGACATGCAGCCAGCTGACTTCCAGAGGAAATGAATCATCC
  AGGCGCAATCTCGGACAGCCTGGTGGCACCTCCCCCGCCGTTCCTCCAGTCTGCCCCATCGTATCACTGA
  CCGCCTCCGGGGCAAAACAGAATCGGGGAGGAATGGGATCCCTCCATCTCGCCAAACCAGAAGAAACCTC
  ACCTGCAGTGAGTCCAGTATGCCCTATCGCCTCACCCGCAGCCTCTCGGTCTAAGCAGCACTGCGGCGTC
  ACCGGGAGTAGCCAAGCTGCACCCAGCAGTTCTTCAGTGGCGCCAGTTGCCAGCCTGTCTGGGGACCTCG
  AAGAGGAGGAGGAGGGCTCCAGAGAAAGCCCCTCACTGCCCAGCTCCAAGAAGGGTGCCGACGAGTTTGA
  GGCCTGGCTGGAGGCACAGGACGCCAACTTCGAAGACGTGCAGAGGGAATTCTCAGGCCTGCGCGTGATC
  GGAGATGAGGATGAAGATGGCTCAGAGGATGGCGAATTCAGTGACCTTGATCTGAGCGACTCTGACCACG
  AGGGGGACGAGGGCGGCGGAGCCGTGGGGGGAGGTAGGTCTCTCCACTCATTGTATAGCCTCTCAGTGAT
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101047 representing YP_001129470
  Red=Cloning sites Green=Tags
  
  MSGQQRGSVILVPEHLAGALTKLMSDFITGQDVTLSGGNIAVKIRDAINQTPGGGDVAILSSLFALWNAL
  PTSGRQSSRDDLIPAAVQALTTAHNLCLGVIPGETSHKDTPESLLRAIVTGLQKLWVDSCGCPECLQCLK
  GLKAIKPGLYEIPRIIPHTKQCSPVNLLNMLVHKLVALRGHVQLAYDARVLTPDFHEIPDLDDSDAVFAR
  TLLAALFHLNMFFILKDYITQDSMSLKQALSGHWMSATGNPLPAAPETLRDYLEAFRNSDNHFYLPTTGP
  LNTFKFPEELLGRVVVIDSSLCAASHVQDVITRGVGAGVPRPQFLALPPAPSRKPQQTCSQLTSRGNESS
  RRNLGQPGGTSPAVPPVCPIVSLTASGAKQNRGGMGSLHLAKPEETSPAVSPVCPIASPAASRSKQHCGV
  TGSSQAAPSSSSVAPVASLSGDLEEEEEGSRESPSLPSSKKGADEFEAWLEAQDANFEDVQREFSGLRVI
  GDEDEDGSEDGEFSDLDLSDSDHEGDEGGGAVGGGRSLHSLYSLSVI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129470
• RefSeq ORF: 1611 bp
• Locus ID: 5176160
• MW: 56.9 kDa