BFRF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101024

Myc-DDK-tagged ORF clone of viral ORF for BFRF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129447

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BFRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101024 represents NCBI reference of YP_001129447 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTCTTTCTTGCTAGACACACGCTGTCAGGAACCGGCGCAGGATGCCATGGCCGGGGTCCCGCTC
  CGGACGTCAGTGAGGTCGACCTTACCCTCCAGGCGTTGGGCGAACGCGGCTTTTCACGACTCCTCGATCT
  CGGCCTCGCTTGCCTCGACCTGAGCTACGTGGAGATGAGGGAATTTGTCGTGTGGGGGAGACCTCCTGCA
  TCCGAGGCCGCCGTGGCATCAACGCCCGGATCACTTTTCAGATCACATTCCTCCGCTTATTGGTTGTCTG
  AAGTGGAACGGCCAGGCGGCTTGGTGAGATGGGCAAGGTCACAAACATCCCCCTCCTCACTGACTTTGGC
  CCCCCACCTTGGTCCCTCTCTGCTTAGCCTGTCCGTGGTTACAGGAGGAGGGTGTGGGGCAGTGGCTTTC
  TGCAATGCCTTCTTTCTGGCGTACTTTCTCGTAGTCCGCTCAGTTTTTCCTGCCTTCAGTGACAGAATCG
  CAGCATGGATATGCGCTCAGTCACCGTTTTGTGAAAACACCAGAGCAGTGGCTCGAGGTTATCGCGGTCT
  GGTGAAACGGTTTCTGGCTTTTGTGTTCGAGCGATCATCCTATGACCCACCACTGCTCCGCCAAAATTCC
  AGACCTGTAGAGCGGTGCTTTGCAATCAAGAACTACGTGCCCGGGCTTGATAGCCAGAGCTGTGTTACAG
  TTCCATCTTTTAGCCGCTGGGCTCAGTCTCATGCTTCCGAACTGGACCCACGGGAGATAAGGGACCGAGT
  CACCCCCGCCACAGCTCCAAGCTTTGTGGCCGATCATGCCTCAGCACTGCTCGCTTCTTTGCAGAAAAAA
  GCAAGTGATACACCATGCGGTAACCCTATTCAGTGGATGTGGTATCGCCTCTTGGTCAACAGCTGCCTGA
  GAAGCGCTCACTGTTTGCTGCCTATCCCCGCTGTGAGTGAAGGAGGGCGGAAGACCGGCGGAGGAGTGGG
  CGAAGATTTGGTGGGCGCAGGTGGCCCTTGCCTTAGTAGGGATGTCTTCGTGGCTATCGTGTCCCGAAAC
  GTGCTGTCATGCCTCCTGAATGTTCCCGCTGCTGGCCCAAGGGCATACAAATGTTTTAGATCTCACGCTT
  CCAGACCAGTGTCTGGTCCTGACTATCCACCTCTGGCAGTGTTCTGTATGGACTGTGGTTACTGCCTCAA
  TTTTGGGAAACAAACTGGGGTAGGAGGTAGACTGAATAGTTTCCGACCGACACTGCAGTTTTACCCACGG
  GATCAAAAGGAGAAGCACGTGCTCACTTGCCACGCTAGTGGGCGGGTGTATTGCTCCAACTGTGGAAGTG
  CCGCCGTGGGATGCCAGAGACTCGCGGAACCCCCCAGCGCCCGATCCGGGTGGAGACCCCGAATCCGCGC
  TGTTCTTCCCCATAACGCTGCCTATGAGCTGGATAGGGGTTCTCGCCTTCTTGATGCTATTATCCCATGT
  CTGGGACCAGACAGAACCTGTATGAGACCCGTCGTACTGCGCGGGGTCACAGTGCGGCAGCTGCTGTACC
  TCACACTGAGGACTGAGGCTCGGGCTGTTTGTTCTATTTGTCAGCAGCGGCAGGCGCCTGAGGATGCCAG
  GGACGAACCCCATTTGTTTTCCAGTTGTCTTGAGGTGGAACTCCCACCCGGGGAGCGGTGCGCTGGCTGT
  AGACTTTATCAGACCAGATACGGGACGCCTGCAGCACAGGCGCACCCACCAGGAGAGGCAGGGGGCGGAT
  TTTCTAGGCAGTCTCCGGCTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101024 representing YP_001129447
  Red=Cloning sites Green=Tags
  
  MALFLARHTLSGTGAGCHGRGPAPDVSEVDLTLQALGERGFSRLLDLGLACLDLSYVEMREFVVWGRPPA
  SEAAVASTPGSLFRSHSSAYWLSEVERPGGLVRWARSQTSPSSLTLAPHLGPSLLSLSVVTGGGCGAVAF
  CNAFFLAYFLVVRSVFPAFSDRIAAWICAQSPFCENTRAVARGYRGLVKRFLAFVFERSSYDPPLLRQNS
  RPVERCFAIKNYVPGLDSQSCVTVPSFSRWAQSHASELDPREIRDRVTPATAPSFVADHASALLASLQKK
  ASDTPCGNPIQWMWYRLLVNSCLRSAHCLLPIPAVSEGGRKTGGGVGEDLVGAGGPCLSRDVFVAIVSRN
  VLSCLLNVPAAGPRAYKCFRSHASRPVSGPDYPPLAVFCMDCGYCLNFGKQTGVGGRLNSFRPTLQFYPR
  DQKEKHVLTCHASGRVYCSNCGSAAVGCQRLAEPPSARSGWRPRIRAVLPHNAAYELDRGSRLLDAIIPC
  LGPDRTCMRPVVLRGVTVRQLLYLTLRTEARAVCSICQQRQAPEDARDEPHLFSSCLEVELPPGERCAGC
  RLYQTRYGTPAAQAHPPGEAGGGFSRQSPAS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129447
• RefSeq ORF: 1773 bp
• Locus ID: 5176228
• MW: 63.9 kDa