BXLF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101073

Myc-DDK-tagged ORF clone of viral ORF for BXLF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129496

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BXLF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101073 represents NCBI reference of YP_001129496 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTTGCTGTGCGTGTTTTGCCTCGTACTCCTCTGGGAAGTGGGCGCCGCATCCCTGAGCGAAGTCA
  AACTGCACCTTGACATCGAGGGGCACGCTTCTCACTACACGATCCCCTGGACCGAGCTGATGGCCAAAGT
  GCCCGGATTGAGCCCTGAGGCACTCTGGCGCGAGGCCAATGTGACAGAAGACCTGGCGTCTATGCTGAAC
  AGGTACAAACTTATTTATAAGACCAGCGGTACACTGGGCATCGCTCTGGCCGAACCCGTGGACATCCCAG
  CAGTCAGCGAGGGGAGCATGCAGGTCGATGCCAGCAAGGTACACCCGGGCGTAATTTCCGGTCTCAATTC
  ACCAGCCTGCATGCTGTCTGCCCCTCTGGAAAAGCAACTTTTCTACTATATTGGTACCATGTTGCCCAAT
  ACCAGACCACATTCTTATGTCTTCTACCAGCTGCGATGCCACCTGTCCTATGTGGCCCTGTCAATCAATG
  GCGACAAATTCCAGTATACAGGTGCCATGACCAGCAAATTCCTGATGGGTACATACAAGCGGGTGACCGA
  AAAAGGTGACGAACATGTTTTGTCACTCATCTTTGGCAAGACTAAAGATCTTCCGGATCTGCGAGGCCCT
  TTTTCTTATCCAAGTCTTACATCAGCCCAGAGCGGAGACTACTCCCTCGTGATTGTTACGACTTTTGTGC
  ACTACGCGAACTTCCACAACTATTTTGTACCCAATCTCAAGGACATGTTCAGCCGAGCCGTGACTATGAC
  CGCCGCATCTTATGCTCGCTATGTCCTTCAAAAGCTGGTGTTGCTTGAGATGAAGGGCGGCTGCCGCGAG
  CCAGAGCTGGACACCGAAACTCTGACTACAATGTTCGAAGTCTCTGTGGCATTCTTCAAAGTCGGACATG
  CCGTGGGTGAAACAGGGAACGGCTGTGTGGATCTCCGGTGGCTTGCAAAAAGCTTTTTCGAATTGACCGT
  CTTGAAGGACATTATCGGCATCTGCTATGGCGCTACCGTGAAGGGCATGCAGAGCTACGGCCTGGAGCGC
  CTCGCAGCCGTGCTGATGGCGACAGTCAAAATGGAAGAACTTGGTCACCTCACCACTGAGAAGCAGGAGT
  ACGCGCTGCGACTGGCTACAGTCGGTTATCCAAAAGCCGGGGTGTACTCCGGTTTGATCGGTGGAGCCAC
  AAGCGTGCTTTTGTCTGCCTACAACAGGCACCCCCTGTTCCAGCCGCTTCATACTGTTATGAGAGAAACC
  CTGTTTATCGGATCACACGTGGTCCTGCGCGAGCTGCGCTTGAATGTCACAACACAGGGACCCAACTTGG
  CCCTGTATCAACTGCTCTCCACTGCACTCTGCAGCGCCCTGGAGATCGGAGAGGTCCTGAGAGGGCTGGC
  ACTCGGGACAGAGAGTGGCTTGTTCAGTCCTTGCTACCTGAGTCTGCGCTTCGACCTCACCCGGGACAAA
  TTGCTGTCAATGGCCCCGCAAGAAGCGATGCTTGATCAGGCTGCTGTCTCAAACGCAGTTGACGGCTTCT
  TGGGACGGTTGAGTTTGGAAAGAGAGGACCGCGACGCCTGGCACCTGCCAGCTTACAAATGTGTGGACAG
  GCTGGACAAGGTTCTGATGATCATCCCGTTGATTAATGTGACATTTATAATTTCAAGCGATAGAGAGGTG
  CGGGGATCCGCCCTTTACGAGGCTTCTACAACATACTTGTCTAGCTCCCTTTTTCTTAGCCCAGTGATTA
  TGAACAAGTGCTCTCAGGGCGCTGTCGCAGGAGAGCCACGCCAGATACCTAAAATACAGAATTTCACAAG
  GACGCAGAAGTCATGTATTTTTTGCGGCTTTGCCCTCCTGTCTTATGATGAAAAAGAAGGACTTGAGACC
  ACTACTTACATCACTAGTCAGGAGGTGCAAAATAGCATATTGTCAAGTAACTATTTTGATTTCGATAATC
  TTCATGTGCATTACTTGCTCCTGACTACTAATGGGACAGTGATGGAAATTGCCGGTTTGTATGAAGAAAG
  GGCTCACGTCGTGCTTGCCATAATCCTGTACTTTATTGCATTCGCCCTTGGTATCTTCCTGGTGCACAAG
  ATCGTGATGTTCTTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101073 representing YP_001129496
  Red=Cloning sites Green=Tags
  
  MQLLCVFCLVLLWEVGAASLSEVKLHLDIEGHASHYTIPWTELMAKVPGLSPEALWREANVTEDLASMLN
  RYKLIYKTSGTLGIALAEPVDIPAVSEGSMQVDASKVHPGVISGLNSPACMLSAPLEKQLFYYIGTMLPN
  TRPHSYVFYQLRCHLSYVALSINGDKFQYTGAMTSKFLMGTYKRVTEKGDEHVLSLIFGKTKDLPDLRGP
  FSYPSLTSAQSGDYSLVIVTTFVHYANFHNYFVPNLKDMFSRAVTMTAASYARYVLQKLVLLEMKGGCRE
  PELDTETLTTMFEVSVAFFKVGHAVGETGNGCVDLRWLAKSFFELTVLKDIIGICYGATVKGMQSYGLER
  LAAVLMATVKMEELGHLTTEKQEYALRLATVGYPKAGVYSGLIGGATSVLLSAYNRHPLFQPLHTVMRET
  LFIGSHVVLRELRLNVTTQGPNLALYQLLSTALCSALEIGEVLRGLALGTESGLFSPCYLSLRFDLTRDK
  LLSMAPQEAMLDQAAVSNAVDGFLGRLSLEREDRDAWHLPAYKCVDRLDKVLMIIPLINVTFIISSDREV
  RGSALYEASTTYLSSSLFLSPVIMNKCSQGAVAGEPRQIPKIQNFTRTQKSCIFCGFALLSYDEKEGLET
  TTYITSQEVQNSILSSNYFDFDNLHVHYLLLTTNGTVMEIAGLYEERAHVVLAIILYFIAFALGIFLVHK
  IVMFFL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129496
• RefSeq ORF: 2118 bp
• Locus ID: 5176171
• MW: 78.3 kDa