BcLF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101070

Myc-DDK-tagged ORF clone of viral ORF for BcLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129493

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BcLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101070 represents NCBI reference of YP_001129493 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCTAACGAGGGGGTGGAAAATCGGCCTTTCCCCTATCTCACTGTCGACGCGGATCTCCTTTCCA
  ACTTGCGCCAGAGCGCAGCTGAAGGCCTCTTTCATTCTTTTGACCTGCTGGTGGGAAAAGATGCAAGGGA
  GGCTGGAATCAAATTTGAAGTTTTGCTCGGGGTTTACACAAATGCGATTCAGTATGTGAGATTTCTTGAG
  ACCGCTCTGGCTGTGTCTTGCGTTAATACTGAGTTTAAAGATCTGAGCAGAATGACCGACGGCAAGATAC
  AGTTTAGAATTAGCGTGCCTACCATAGCACACGGCGATGGCAGAAGGCCCTCAAAGCAGCGCACCTTCAT
  CGTGGTGAAGAACTGTCACAAACACCATATTTCCACCGAGATGGAACTGAGCATGCTGGATCTCGAGATA
  CTGCACTCCATTCCAGAAACCCCCGTCGAGTATGCCGAATACGTTGGAGCAGTAAAAACAGTTGCTAGCG
  CCCTCCAGTTCGGTGTTGACGCACTCGAGAGAGGACTTATAAACACAGTGCTGTCAGTGAAGCTCAGACA
  CGCACCACCCATGTTTATATTGCAGACACTGGCGGATCCGACTTTCACGGAGAGAGGATTTAGCAAGACC
  GTGAAGAGCGATCTTATCGCCATGTTCAAGCGCCACCTCCTTGAGCACAGCTTCTTCCTGGATAGAGCTG
  AGAATATGGGGTCTGGGTTCTCACAATACGTACGATCTCGCCTGAGCGAGATGGTTGCAGCCGTGTCAGG
  TGAAAGCGTGCTGAAGGGCGTCTCTACTTACACAACTGCCAAGGGTGGCGAACCCGTCGGAGGGGTGTTT
  ATCGTAACCGACAATGTGCTGCGCCAGCTGCTGACCTTTCTTGGTGAAGAGGCTGATAACCAGATCATGG
  GTCCCTCCTCCTATGCCAGCTTTGTTGTTAGGGGCGAGAACCTCGTAACTGCTGTGTCCTATGGTAGGGT
  GATGCGAACATTCGAACACTTCATGGCCAGGATTGTCGATTCTCCCGAGAAAGCCGGATCAACCAAGTCC
  GACTTGCCTGCTGTTGCCGCAGGCGTTGAGGATCAGCCCAGGGTGCCTATATCTGCCGCCGTGATTAAAC
  TGGGGAATCATGCTGTTGCTGTGGAGTCCCTGCAAAAGATGTATAATGACACTCAATCCCCCTACCCACT
  GAACAGGCGAATGCAGTACAGCTATTACTTTCCTGTCGGACTGTTCATGCCTAACCCTAAGTACACTACA
  AGTGCGGCTATAAAGATGCTTGACAATCCCACCCAGCAACTGCCTGTGGAGGCATGGATCGTTAATAAAA
  ATAACCTGCTGCTCGCCTTCAATCTCCAGAACGCACTCAAAGTCTTGTGTCACCCTCGCCTCCATACACC
  TGCCCACACCCTTAATTCCCTGAATGCTGCACCCGCTCCGAGAGACCGAAGGGAAACTTACAGTCTGCAA
  CATAGGAGACCCAACCACATGAACGTCCTTGTCATCGTGGATGAGTTCTATGACAACAAGTACGCTGCAC
  CAGTTACAGACATTGCACTTAAGTGTGGGCTTCCAACAGAGGATTTCCTGCACCCCTCTAATTACGACCT
  CCTTCGCCTGGAGCTCCATCCTCTTTACGATATATACATCGGACGGGACGCCGGCGAAAGGGCTCGGCAC
  AGAGCTGTGCATCGCCTGATGGTGGGTAATTTGCCCACACCCCTGGCCCCAGCGGCCTTCCAGGAGGCCA
  GAGGACAGCAATTTGAGACCGCCACCAGCCTGGCACACGTCGTAGACCAGGCTGTAATCGAAACGGTGCA
  GGATACAGCATATGATACCGCCTACCCGGCCTTTTTTTATGTTGTGGAGGCCATGATCCACGGTTTCGAG
  GAAAAGTTCGTGATGAACGTTCCGCTGGTCTCTCTTTGCATCAACACATACTGGGAAAGAGCGGGACGGC
  TTGCCTTCGTAAATAGCTTCAGCATGATTAAGTTCATCTGCCGACACCTCGGCAACAACGCTATATCCAA
  GGAGGCCTATTCCATGTACAGGAAGATTTACGGTGAACTGATTGCCCTCGAGCAAGCCCTCATGCGACTC
  GCCGGCTCAGACGTTGTTGGCGACGAGTCCGTTGGCCAATACGTTTGTGCACTGCTTGATCCCAACCTGC
  TTCCACCCGTGGCTTATACGGACATCTTTACCCACCTGCTTACTGTGAGTGATCGCGCCCCCCAAATTAT
  CATAGGAAATGAGGTTTACGCCGACACACTGGCCGCGCCCCAGTTCATCGAAAGGGTTGGGAACATGGAT
  GAGATGGCTGCTCAGTTTGTGGCGCTCTACGGCTACCGCGTCAATGGAGATCATGACCATGACTTCCGAC
  TGCATCTTGGCCCTTACGTGGACGAAGGACACGCGGATGTGTTGGAAAAAATATTTTACTACGTCTTCCT
  GCCCACATGTACCAACGCGCATATGTGCGGGCTGGGTGTAGATTTCCAGCACGTGGCTCAAACTCTCGCA
  TACAATGGCCCGGCTTTTAGCCACCATTTCACTAGGGATGAGGATATTCTGGATAACCTTGAAAACGGCA
  CTTTGAGAGATCTCTTGGAGATCAGTGATCTTCGACCGACCGTAGGCATGATACGCGATCTGAGTGCCTC
  CTTTATGACTTGTCCAACCTTTACTAGAACCGTGCGGGTGAGCGTGGATAATGATGTAACCCAGCAGCTG
  GCTCCTAACCCTGCTGATAAGCGCACGGAGCAGACAGTCCTGGTTAACGGGCTGGTGGCCTTCGCCTTCA
  GCGAGCGAACCCGCGCAGTGACCCAGTGTCTGTTTCACGCGATCCCATTCCACATGTTCTACGGAGACCC
  TCGCGTCGCCGCAACCATGCACCAGGATGTGGCAACTTTTGTGATGCGGAATCCGCAGCAGCGGGCTGTG
  GAAGCATTCAACCGACCAGAGCAACTTTTCGCCGAGTACAGGGAGTGGCACAGGTCCCCAATGGGCAAGT
  ACGCCGCTGAGTGTCTTCCCTCCTTGGTGAGCATCTCAGGGATGACCGCAATGCATATCAAGATGAGCCC
  CATGGCGTACATTGCCCAGGCGAAATTGAAGATCCACCCCGGAGTGGCTATGACGGTAGTTAGAACTGAT
  GAGATCCTGAGTGAAAACATACTGTTCTCCTCCCGGGCCTCAACAAGTATGTTCATCGGGACCCCAAACG
  TGAGTCGCCGGGAGGCAAGAGTTGACGCAGTAACGTTTGAGGTACACCACGAAATGGCAAGCATAGACAC
  TGGGCTGTCCTATTCTTCCACAATGACACCTGCGCGGGTAGCCGCCATCACAACTGACATGGGCATTCAT
  ACACAGGATTTCTTTTCTGTTTTTCCCGCCGAGGCCTTCGGGAATCAGCAGGTCAATGACTATATTAAAG
  CAAAAGTAGGAGCGCAGCGAAACGGTACTCTGCTGCGGGACCCCCGGACTTACCTCGCCGGCATGACCAA
  CGTTAACGGAGCACCTGGACTGTGTCATGGACAGCAGGCCACTTGCGAAATCATTGTCACCCCAGTCACT
  GCAGATGTCGCGTACTTTCAAAAGTCCAACTCCCCACGCGGCAGAGCTGCTTGCGTTGTTTCCTGCGAAA
  ACTACAATCAAGAAGTGGCCGAAGGGCTGATTTATGATCACAGCCGACCCGATGCTGCCTATGAATATCG
  GAGTACCGTAAACCCTTGGGCATCTCAGCTGGGGAGCCTGGGAGATATCATGTATAACTCAAGCTATAGG
  CAGACAGCTGTGCCAGGTCTGTACTCTCCATGTCGCGCTTTCTTTAACAAGGAGGAACTGCTGCGGAATA
  ACCGGGGACTCTACAATATGGTCAACGAATACTCCCAGAGGCTGGGCGGGCACCCTGCTACTAGTAATAC
  AGAGGTCCAATTTGTAGTGATCGCCGGGACCGACGTGTTCTTGGAACAACCCTGCTCATTTCTGCAGGAG
  GCATTCCCCGCCCTGTCCGCAAGCAGTCGGGCTCTGATAGACGAGTTCATGTCCGTGAAACAGACCCACG
  CCCCCATTCACTATGGACACTATATCATTGAGGAGGTAGCACCCGTGAGACGAATTCTCAAGTTCGGCAA
  CAAGGTTGTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101070 representing YP_001129493
  Red=Cloning sites Green=Tags
  
  MASNEGVENRPFPYLTVDADLLSNLRQSAAEGLFHSFDLLVGKDAREAGIKFEVLLGVYTNAIQYVRFLE
  TALAVSCVNTEFKDLSRMTDGKIQFRISVPTIAHGDGRRPSKQRTFIVVKNCHKHHISTEMELSMLDLEI
  LHSIPETPVEYAEYVGAVKTVASALQFGVDALERGLINTVLSVKLRHAPPMFILQTLADPTFTERGFSKT
  VKSDLIAMFKRHLLEHSFFLDRAENMGSGFSQYVRSRLSEMVAAVSGESVLKGVSTYTTAKGGEPVGGVF
  IVTDNVLRQLLTFLGEEADNQIMGPSSYASFVVRGENLVTAVSYGRVMRTFEHFMARIVDSPEKAGSTKS
  DLPAVAAGVEDQPRVPISAAVIKLGNHAVAVESLQKMYNDTQSPYPLNRRMQYSYYFPVGLFMPNPKYTT
  SAAIKMLDNPTQQLPVEAWIVNKNNLLLAFNLQNALKVLCHPRLHTPAHTLNSLNAAPAPRDRRETYSLQ
  HRRPNHMNVLVIVDEFYDNKYAAPVTDIALKCGLPTEDFLHPSNYDLLRLELHPLYDIYIGRDAGERARH
  RAVHRLMVGNLPTPLAPAAFQEARGQQFETATSLAHVVDQAVIETVQDTAYDTAYPAFFYVVEAMIHGFE
  EKFVMNVPLVSLCINTYWERAGRLAFVNSFSMIKFICRHLGNNAISKEAYSMYRKIYGELIALEQALMRL
  AGSDVVGDESVGQYVCALLDPNLLPPVAYTDIFTHLLTVSDRAPQIIIGNEVYADTLAAPQFIERVGNMD
  EMAAQFVALYGYRVNGDHDHDFRLHLGPYVDEGHADVLEKIFYYVFLPTCTNAHMCGLGVDFQHVAQTLA
  YNGPAFSHHFTRDEDILDNLENGTLRDLLEISDLRPTVGMIRDLSASFMTCPTFTRTVRVSVDNDVTQQL
  APNPADKRTEQTVLVNGLVAFAFSERTRAVTQCLFHAIPFHMFYGDPRVAATMHQDVATFVMRNPQQRAV
  EAFNRPEQLFAEYREWHRSPMGKYAAECLPSLVSISGMTAMHIKMSPMAYIAQAKLKIHPGVAMTVVRTD
  EILSENILFSSRASTSMFIGTPNVSRREARVDAVTFEVHHEMASIDTGLSYSSTMTPARVAAITTDMGIH
  TQDFFSVFPAEAFGNQQVNDYIKAKVGAQRNGTLLRDPRTYLAGMTNVNGAPGLCHGQQATCEIIVTPVT
  ADVAYFQKSNSPRGRAACVVSCENYNQEVAEGLIYDHSRPDAAYEYRSTVNPWASQLGSLGDIMYNSSYR
  QTAVPGLYSPCRAFFNKEELLRNNRGLYNMVNEYSQRLGGHPATSNTEVQFVVIAGTDVFLEQPCSFLQE
  AFPALSASSRALIDEFMSVKQTHAPIHYGHYIIEEVAPVRRILKFGNKVVF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 4143 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129493
• RefSeq ORF: 4143 bp
• Locus ID: 5176226
• MW: 153.9 kDa