BGLF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101062

Myc-DDK-tagged ORF clone of viral ORF for BGLF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129486

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BGLF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101062 represents NCBI reference of YP_001129486 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCCGCGGCCAACAGTAGCCGAGAGCAGCTGCGGAAGTTCCTGAACAAGGAGTGCCTGTGGGTGT
  TGTCCGACGCTTCCACCCCCCAGATGAAGGTGTACACCGCAACAACTGCTGTCTCTGCTGTATACGTGCC
  TCAGATTGCAGGACCACCCAAGACATACATGAATGTCACCCTGATCGTGTTGAAGCCTAAGAAGAAACCC
  ACATACGTGACAGTGTATATAAACGGCACACTGGCAACAGTCGCTCGGCCAGAGGTTCTGTTTACTAAAG
  CTGTCCAGGGACCTCACTCCCTGACCCTTATGTACTTTGGCGTCTTTAGCGATGCCGTGGGTGAGGCTGT
  GCCGGTGGAAATCCGGGGTAACCCCGTCGTTACCTGTACCGACCTTACAACAGCCCACGTGTTCACAACA
  TCAACCGCGGTAAAAACAGTGGAGGAACTCCAGGACATTACCCCCTCCGAAATTATCCCTCTGGGCAGGG
  GAGGCGCATGGTACGCGGAAGGCGCGCTCTATATGTTTTTTGTCAATATGGACATGCTCATGTGCTGCCC
  AAATATGCCCACATTCCCTAGTCTGACCCATTTCATCAACCTGCTGACTCGGTGTGACAATGGCGAATGT
  GTCACCTGCTATGGGGCAGGCGCCCACGTCAATATCTTGCGCGGCTGGACAGAGGATGATTCCCCAGGTA
  CCTCCGGAACCTGTCCCTGCCTGCTGCCATGCACCGCATTGAACAACGATTATGTGCCTATCACCGGCCA
  CCGCGCCTTGCTCGGCCTGATGTTTAAGCCAGAAGATGCACCTTTCGTGGTTGGTCTCAGGTTCAACCCT
  CCTAAAATGCATCCTGATATGAGTCGCGTGCTCCAGGGGGTGTTGGCCAACGGAAAGGAGGTGCCCTGCA
  CCGCACAGCCCTGGACTCTGCTGCGCTTCTCTGACCTGTATTCAAGGGCAATGCTGTACAATTGTCAGGT
  ACTGAAGAGACAGGTATTGCACAGCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101062 representing YP_001129486
  Red=Cloning sites Green=Tags
  
  MASAANSSREQLRKFLNKECLWVLSDASTPQMKVYTATTAVSAVYVPQIAGPPKTYMNVTLIVLKPKKKP
  TYVTVYINGTLATVARPEVLFTKAVQGPHSLTLMYFGVFSDAVGEAVPVEIRGNPVVTCTDLTTAHVFTT
  STAVKTVEELQDITPSEIIPLGRGGAWYAEGALYMFFVNMDMLMCCPNMPTFPSLTHFINLLTRCDNGEC
  VTCYGAGAHVNILRGWTEDDSPGTSGTCPCLLPCTALNNDYVPITGHRALLGLMFKPEDAPFVVGLRFNP
  PKMHPDMSRVLQGVLANGKEVPCTAQPWTLLRFSDLYSRAMLYNCQVLKRQVLHSY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129486
• RefSeq ORF: 1008 bp
• Locus ID: 5176204
• MW: 36.9 kDa