nef protein (NC_001722) Virus Tagged ORF Clone

SKU VC101750

Myc-DDK-tagged ORF clone of viral ORF for nef protein [Human immunodeficiency virus 2], codon optimized for human cell expression, NP_056845

Product Data

• Type: Virus Tagged ORF Clone
• Target Symbol: nef protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101750 represents NCBI reference of NP_056845 with codon optimized for human cell expression ; Red=Cloning site Blue=ORF Green=Tags(s); ; GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGAGCCTCCGGATCCAAAAAACTGTCAAAACATAGCCGCGGGCTGAGAGAGAGACTGCTGAGAGCAA; GAGGGGACGGCTACGGGAAGCAGCGCGACGCCAGCGGAGGGGAGTATTCTCAGTTTCAAGAAGAGTCAGG; CCGAGAACAGAATTCACCCTCCTGCGAAGGCCAACAATACCAGCAGGGGGAATACATGAACTCCCCATGG; CGGAACCCAGCCACCGAAAGGCAGAAGGACCTTTATAGACAACAGAACATGGACGATGTGGATAGCGACG; ACGACGATCTCATCGGCGTCCCCGTGACACCTCGGGTTCCTCGGCGAGAAATGACATATAAGCTGGCAAT; CGATATGAGCCACTTCATTAAGGAAAAAGGGGGTCTCCAGGGAATGTTTTATTCAAGGAGGAGACACAGA; ATATTGGATATCTACCTCGAGAAGGAAGAGGGAATCATTCCGGACTGGCAAAACTACACCCATGGACCAG; GGGTGAGGTACCCAATGTACTTTGGCTGGCTTTGGAAGCTTGTGAGCGTGGAACTTTCTCAGGAAGCCGA; AGAGGACGAGGCAAATTGTTTGGTGCACCCAGCACAGACATCTAGGCATGATGACGAACACGGGGAGACA; CTCGTATGGCAGTTCGATTCTATGCTTGCCTATAACTACAAAGCTTTTACCCTGTATCCCGAGGAATTCG; GCCACAAATCCGGCCTGCCAGAAAAGGAATGGAAAGCTAAATTGAAGGCCCGAGGCATACCCTACAGTGA; G; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101750 representing NP_056845 ; Red=Cloning sites Green=Tags; ; MGASGSKKLSKHSRGLRERLLRARGDGYGKQRDASGGEYSQFQEESGREQNSPSCEGQQYQQGEYMNSPW; RNPATERQKDLYRQQNMDDVDSDDDDLIGVPVTPRVPRREMTYKLAIDMSHFIKEKGGLQGMFYSRRRHR; ILDIYLEKEEGIIPDWQNYTHGPGVRYPMYFGWLWKLVSVELSQEAEEDEANCLVHPAQTSRHDDEHGET; LVWQFDSMLAYNYKAFTLYPEEFGHKSGLPEKEWKAKLKARGIPYSE; ; TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001722
• ORF Size: 771 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NC_001722.1, NP_056845
• RefSeq ORF: 771 bp
• Locus ID: 1724715
• MW: 29.9 kDa