pCas-Guide-CRISPRa gene activation vector

CAT#: GE100055

pCas-Guide-CRISPRa vector, All-in-one Vector for CRISPR/Cas9 Gene activation with dCas9-VP64 and gRNA cloning site.

Product images

Specifications

Product Data

• Function: All-in-one CRISPRa SAM vector
• E. coli Selection: Ampicillin (100 ug/mL)
• Features:
  • BamH I and BsmB I can be used for genomic target sequence cloning
  • gRNA under U6 promoter
  • gRNA is modified with MS2 RNA aptamer in the loops
  • CMV driven dCas9-VP64, which can activate gene expression
  • For robust gene activation, needs to work with pCRISPRa-Enhancer
  • CF3 primer can be used to sequence target sequence after cloning
  • Download vector sequence