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pLenti-EF1a-Cas-Guide

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SKU
GE100045
pLenti-EF1a-Cas-Guide, (with EF1a-Cas9) for gRNA target sequence cloning (10 µg)
  $880.00
In Stock*

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Specifications
Specifications
Product Data
Function All-in-One CRISPR/Cas9 vectors
Features
  • Lentiviral vector, ideal for hard-to-transfect and animal models
  • All-in-one vector
    1. Expresses human codon-optimized Cas9, C-terminal Myc-DDK tagged
    2. BamH I and BsmB I can be used for genomic target sequence cloning
    3. gRNA expression driven by U6 promoter
    4. Lenti particles can be produced using the high efficient lenti packaging kit
Download Vector Sequence
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

Neuronal FSTL4 negatively regulates BDNF-mediated neuron-glioma interaction
Sun, Xiao, Wang et al
Neurochem Int (2025) 191, 106072 DOI: 10.1016/j.neuint.2025.106072
Cited by 2
AI Summary: OriGene’s pLenti-EF1a-Cas-Guide (GE100045) plasmid was used to clone sgRNAs for CRISPR knockout of endogenous FSTL4 in mouse neurons, enabling the study of FSTL4’s role in regulating BDNF-mediated neuron-glioma interactions.
Snippet: For CRISPR knockout of endogenous FSTL4 in mouse cultured neurons, 2 independent sgRNAs were designed using synthego website (https://design.synthego.com/#/). Each sgRNA was cloned into pLenti-EF1a-Cas-Guide (GE100045, Origene). The sgRNA sequences against mouse FSTL4 are as follows
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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