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pLenti-Guide-Puro

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SKU
GE100032
pLenti-Guide-Puro, gRNA cloning vector in lenti vector with puromycin as mammalian selection marker (10ug)
  $880.00
In Stock*

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Specifications
Specifications
Product Data
Function gRNA only vectors
Features

gRNA only vector in lenti backbone

  • gRNA expression under U6 promoter
  • 3rd generation Lenti vector, ideal for hard-to-transfect cells
  • BamH1 and BsmB I sites are used for target sequence cloning
  • Puro resistant gene allows puro selection in both transfected and transduced cells
  • Lenti particles can be produced using high efficiency lenti packaging kits
  • CF3 primer can be used to sequence the target sequence after cloning
  • E. Coli selection: chloramphenicol (34 ug/mL)3rd generation Lenti vector, ideal for hard-to-transfect cells
Download Vector Sequence
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

Epigenetic dysregulation of the H3K14ac/H3K9me2 axis is associated with malignant transformation and prognosis in colon adenocarcinoma
Roberti, Tejedor, Martínez-Sánchez et al
researchsquare.com (2026)
AI Summary: OriGene's pLenti-Guide-Puro vectors (GE100032) were used as cloning backbones for sgRNAs targeting EHMT2 and HDAC7, enabling gene editing experiments in the study of epigenetic regulation in colon adenocarcinoma.
Snippet: … Forward and reverse primers containing the sgRNA sequences targeting EHMT2 and HDAC7 (Additional File 3 – Table S2), along with flanking adaptor sequences, were annealed and cloned into BamHI/BsmBI-linearized pLenti-Guide-Puro vectors (Origene, GE100032) using the In-Fusion HD Cloning Kit (Takara, #638911), according to the manufacturer’s instructions
Sodium arsenite-induced DNA methylation alterations exacerbated by p53 knockout in MCF7 cells
Chung, Khoueiry, Sallé et al
Heliyon (2024) 10 (21), e39548 DOI: 10.1016/j.heliyon.2024.e39548
Cited by 2
AI Summary: OriGene's pLenti-Guide-Puro (#GE100032) CRISPR vector was used to deliver guide RNAs targeting p53 into MCF7 cells, enabling gene knockout experiments in the study.
Snippet: Guide RNA targeting p53 were designed using the ThermoFisher GeneArt CRISPR design tool (sequences in Supplemental Table 1) and ligated into the pLenti-Guide-Puro (#GE100032, Origene) CRISPR vector, and positive clones confirmed by Sanger sequencing (Eurofins Genomics). …
Nicotinamide N-methyltransferase (NNMT) regulates the glucocorticoid signaling pathway during the early phase of adipogenesis
Roberti, Tejedor, Díaz-Moreno et al
Sci Rep (2023) 13 (1), 8293 DOI: 10.1038/s41598-023-34916-z
Cited by 10
AI Summary: OriGene’s pLenti-Guide-Puro vector (GE100032) was used to clone guide RNAs for CRISPR/Cas9-mediated knockout of NNMT, enabling the study of NNMT’s role in adipogenesis in this paper.
Snippet: iously described28 adding a flanking region for proper cloning, using the In-Fusion HD Cloning Kit (Takara). These oligonucleotides were cloned in a BamH1-BsmB1 linearized pLenti-Guide-Puro vector (Origene, GE100032) using the In-Fusion HD Cloning system following the manufacturer’s recommendations. A two-vector approach was used to deliver Cas9 and the gRNAs to the cells. To assemble functi
FigureFigureFigure
Exploiting the metabolic dependencies of the broad amino acid transporter SLC6A14
Dejure, Butzer, Lindemann et al
Oncotarget (2020) 11 (48), 4490-4503 DOI: 10.18632/oncotarget.27758
Cited by 6
FigureFigureFigure
Exploiting the metabolic dependencies of the broad amino acid transporter SLC6A14
Dejure, Butzer, Lindemann et al
Oncotarget (2020) 11 (48), 4490-4503 DOI: 10.18632/oncotarget.27758
Cited by 6
Snippet: To induce Cas9 expression, cells were treated with 1 μg/ml doxycycline (AppliChem GmbH, A2951.0025) and 24 h later transfected with pLenti-Guide-Puro vectors (Origene, {"type":"entrez-nucleotide","attrs":{"text":"GE100032","term_id":"208302827","term_text":"GE100032
Showing 1-5 of 5 papers.
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