pLenti-Guide-Puro

CAT#: GE100032

pLenti-Guide-Puro, gRNA cloning vector in lenti vector with puromycin as mammalian selection marker (10ug)


Reconstitution Protocol

USD 850.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
    • 500 pmol

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Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Specifications

Product Data
Function gRNA only vectors
Features

gRNA only vector in lenti backbone

  • gRNA expression under U6 promoter
  • 3rd generation Lenti vector, ideal for hard-to-transfect cells
  • BamH1 and BsmB I sites are used for target sequence cloning
  • Puro resistant gene allows puro selection in both transfected and transduced cells
  • Lenti particles can be produced using high efficiency lenti packaging kits
  • CF3 primer can be used to sequence the target sequence after cloning
  • E. Coli selection: chloramphenicol (34 ug/mL)3rd generation Lenti vector, ideal for hard-to-transfect cells

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.