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pLenti-Cas9-P2A-tGFP

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SKU
GE100031
pLenti-Cas9-P2A-tGFP, Cas9 in lenti vector with tGFP after P2A (10 ug)
  $880.00
In Stock*

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Specifications
Specifications
Product Data
Function Cas9 only vectors
Features

Cas9 only vector with GFP expression in lenti backbone

  • 3rd generation Lenti vector, ideal for hard-to-transfect cells
  • Cas9 under CMV promoter for mammalian expression after transfection or transduction
  • P2A-tGFP is within the Lentiviral packaging cassette and would allow GFP expression in both the transfected and the transduced cells.
  • Lenti particles can be produced using high efficiency lenti packaging kits
Download Vector Sequence
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

Tumor Suppressive Role of MUC6 in Wilms Tumor via Autophagy-Dependent β-Catenin Degradation
Liu, Liu, Zhang et al
Front Oncol (2022) 12, 756117 DOI: 10.3389/fonc.2022.756117
Cited by 9
AI Summary: OriGene’s pLenti-Cas9-P2A-tGFP vector was used to perform CRISPR-mediated mutation of the MUC6 gene in WiT49 cells, enabling the study of MUC6’s tumor-suppressive role in Wilms tumor.
Snippet: packaged and amplified in HEK293T cells. Cell lines were infected at an MOI of 5. CRISPR-Mediated Mutation of MUC6 CRISPR-mediated editing in WiT49 cell was performed with pLenti-Cas9-P2A-tGFP from Origene and gRNAs targeting indicated sites were cloned into this vector. WiT49 cells were infected by pLenti-Cas9-P2A-tGFP with targeting gRNA or scramble control in the presence of donor DNA for 72
FigureFigureFigure
Showing 1-1 of 1 papers.
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