HNF 4 alpha(HNF4A) (NM_000457) Human Recombinant Protein

CAT#: TP317863

Recombinant protein of human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2

Bulk Request/Custom Protein Development


 Product Datasheet for 'TP317863'

USD 438.00

USD 730.00


Availability*
In Stock

Size
    • 20 ug

Product images

Specifications

Product Data
Description Recombinant protein of human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC217863. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag C-MYC/DDK
Predicted MW 52.6 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at –80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Bioactivity HNF4A Activity Verified in a DNA-binding Assay: HNF4A (TP317863, transcript variant 2) activity was measured in a colorimetric DNA-binding assay. Purified HNF4A protein containing a C-terminal MYC/DDK tag was incubated with biotinylated double-stranded oligonucleotide containing the HNF4A consensus DNA-binding sequence (see below). Following incubation, the reaction was transferred to a streptavidin-coated microplate to allow capture of the DNA-protein complex. After washing, the captured protein was detected with an anti-DDK peroxidase conjugate and colorimetric signal detection with TMB. Specificity of the protein-DNA interaction was confirmed by carrying out the binding in the presence of an unlabeled competitor oligonucleotide and by comparison to binding to an oligonucleotide containing a mutation in the consensus binding sequence.
Reference Data
RefSeq NP_000448
Locus ID 3172
Refseq Size 4737
Cytogenetics 20q13.12
Refseq ORF 1422
Synonyms FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14
Summary Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. [UniProtKB/Swiss-Prot Function]
Protein Families
Protein Pathways
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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