c-Myc(MYC) (NM_002467) Human Recombinant Protein

CAT#: TP301611

Recombinant protein of human v-myc myelocytomatosis viral oncogene homolog (avian) (MYC)

Product images

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  Boxplots of the reactivities of the autoantibodies in ELISA against full-length C-MYC protein (OriGene TP301611) in the sera of prostate cancer cases and healthy controls. Panel F shows the ROC curve for C-MYC. Figure cited from Genes Cancer, PMID: 28191285

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  ELISA dose-response curves for the inhibition of the interaction between Max and Myc (OriGene TP301611) by Myc inhibitors N12, C12, E07, or two of their combinations. The data are represented as a fraction of activity compared to a DMSO-treated control sample and are plotted as a mean of 2 - 5 experiments +- SD. The X-axis refers to the concentration of each monomer used. Figure cited from PLoS ONE, PMID: 25875098

Specifications

Product Data

• Description: Recombinant protein of human v-myc myelocytomatosis viral oncogene homolog (avian) (MYC)
• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: Recombinant protein was produced with TrueORF clone, RC201611. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-MYC/DDK
• Predicted MW: 50.4 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at –80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
• Bioactivity: ELISA binding assay (PMID: 25875098)
  EMSA assay (PMID: 25892221)
  ELISA capture for autoantibodies (PMID: 28191285)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Reference Data

• Locus ID: 4609
• Refseq Size: 2379
• Cytogenetics: 8q24.21
• Refseq ORF: 1365
• Synonyms: bHLHe39; c-Myc; MRTL; MYCC
• Summary: This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
• Protein Families: Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Stem cell - Pluripotency, Embryonic stem cells, Induced pluripotent stem cells, Transcription Factors, Druggable Genome
• Protein Pathways: MAPK signaling pathway, ErbB signaling pathway, Cell cycle, Wnt signaling pathway, TGF-beta signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, Colorectal cancer, Endometrial cancer, Thyroid cancer, Bladder cancer, Chronic myeloid leukemia, Acute myeloid leukemia, Small cell lung cancer