Acid sphingomyelinase (SMPD1) Human Gene Knockout Kit (CRISPR)

SKU KN219758LP

SMPD1 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Vector: Luciferase-Puro
• Target Symbol: Acid sphingomyelinase
• Locus ID: 6609
• Components:

  KN219758G1, Acid sphingomyelinase gRNA vector 1 in pCas-Guide CRISPR vector

  KN219758G2, Acid sphingomyelinase gRNA vector 2 in pCas-Guide CRISPR vector

  KN219758LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000543, NM_001007593, NM_001318087, NM_001318088, NR_027400, NR_134502, NM_001365135
• UniProt ID: P17405
• Synonyms: ASM; ASMASE; NPD
• Summary: The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]
• Shipping: Ambient