GBA Human Gene Knockout Kit (CRISPR)

CAT#: KN201614

GBA - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: GBA
• Locus ID: 2629
• Components:

  KN201614G1, GBA gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CAGCATTGTCACAGTGCTTC

  KN201614G2, GBA gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTTTTCAAGTCCTTCCAGAG

  KN201614D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TAGGGCAAAG ACTGTCTTAA ACATCTTGGT AGTGTCAGTA TTTTGCACAG TGAAGTTTTT TTTTTTAAAT TATATCAGCT TTATTTGTAC CTTTTTGACA TTTCTATCAA AAAAGAAGTG TGCCTGCTGT GGTTCCCATC CTCTGGGATT TAGGAGCCTC TACCCCATTC TCCATGCAAA TCTGTGTTCT AGGCTCTTCC TAAAGTTGTC ACCCATACAT GCCCTCCAGA GTTTTATAGG GCATATAATC TGTAACAGAT GAGAGGAAGC CAATTGCCCT TTAGAAATAT GGCTGTGATT GCCTCACTTC CTGTGTCATG TGACGCTCCT AGTCATCACA TGACCCATCC ACATCGGGAA GCCGGAATTA CTTGCAGGGC TAACCTAGTG CCTATAGCTA AGGCAGGTAC CTGCATCCTT GTTTTTGTTT AGTGGATCCT CTATCCTTCA GAGACTCTGG AACCCCTGTG GTCTTCTCTT CATCTAATGA CCCTGAGGGG TGGAATCCTG GCACTGGAAT TTAATGAATG ACAGACTCTC TTTGAATCCA GGGCCATCAT GGCTCTTTGA GCAAGGCACA GATGGAGGGA GGGGTCGAAG TTGAAATGGG TGGGAAGAGT GGTGGGGAGC ATCCTGATTT GGGGTGGGCA GAGAGTTGTC ATCAGAAGGG TTGCAGGGAG AGCTGCACCC AGGTTTCTGT GGGCCTTGTC CTAATGAATG TGGGAGACCG GGCCATGGGC ACCCAAAGGC AGCTAAGCCC TGCCCAGGAG AGTAGTTGAG GGGTGGAGAG GGGCTTGCTT TTCAGTCATT CCTCATTCTG TCCTCAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGAG TGAGTCAAGG CAGTGGGGAG GTAGCACAGA GCCTCCCTTC TGCCTCATAG TCCTTTGGTA GCCTTCCAGT AAGCTGGTGG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000157, NM_001005741, NM_001005742, NM_001005749, NM_001005750, NM_001171811, NM_001171812
• UniProt ID: P04062
• Synonyms: GBA1; GCB; GLUC
• Summary: This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]