Human C2orf60 (TYW5) activation kit by CRISPRa

SKU GA115082

TYW5 CRISPRa kit - CRISPR gene activation of human tRNA-yW synthesizing protein 5

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: TYW5
• Locus ID: 129450
• Components:

  GA115082G1, C2orf60 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA115082G2, C2orf60 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA115082G3, C2orf60 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001039693, NM_152382, NR_004862, NR_109905, NR_109906, NR_109907
• UniProt ID: A2RUC4
• Synonyms: C2orf60; hTYW5
• Summary: tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes.[UniProtKB/Swiss-Prot Function]