Human Methionine Sulfoxide Reductase B (MSRB1) activation kit by CRISPRa

SKU GA109966

MSRB1 CRISPRa kit - CRISPR gene activation of human methionine sulfoxide reductase B1

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: MSRB1
• Locus ID: 51734
• Components:

  GA109966G1, Methionine Sulfoxide Reductase B gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA109966G2, Methionine Sulfoxide Reductase B gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA109966G3, Methionine Sulfoxide Reductase B gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016332
• UniProt ID: Q9NZV6
• Synonyms: HSPC270; SELR; SELX; SepR; SEPX1
• Summary: The protein encoded by this gene belongs to the methionine-R-sulfoxide reductase B (MsrB) family. Members of this family function as repair enzymes that protect proteins from oxidative stress by catalyzing the reduction of methionine-R-sulfoxides to methionines. This protein is highly expressed in liver and kidney, and is localized to the nucleus and cytosol. It is the only member of the MsrB family that is a selenoprotein, containing a selenocysteine (Sec) residue at its active site. It also has the highest methionine-R-sulfoxide reductase activity compared to other members containing cysteine in place of Sec. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A pseudogene of this locus has been identified on chromosome 19. [provided by RefSeq, Aug 2017]