Human Nephrin (NPHS1) activation kit by CRISPRa

SKU GA103252

NPHS1 CRISPRa kit - CRISPR gene activation of human NPHS1 adhesion molecule, nephrin

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: NPHS1
• Locus ID: 4868
• Components:

  GA103252G1, Nephrin gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103252G2, Nephrin gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103252G3, Nephrin gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004646
• UniProt ID: O60500
• Synonyms: CNF; nephrin; NPHN
• Summary: This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]