Human GTP cyclohydrolase 1 (GCH1) activation kit by CRISPRa

SKU GA101762

GCH1 CRISPRa kit - CRISPR gene activation of human GTP cyclohydrolase 1

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: GCH1
• Locus ID: 2643
• Components:

  GA101762G1, GTP cyclohydrolase 1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101762G2, GTP cyclohydrolase 1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101762G3, GTP cyclohydrolase 1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000161, NM_001024024, NM_001024070, NM_001024071
• UniProt ID: P30793
• Synonyms: DYT5; DYT5a; DYT14; GCH; GTP-CH-1; GTPCH1; HPABH4B
• Summary: This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme. [provided by RefSeq, Jul 2008]