BcLF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101157

Myc-DDK-tagged ORF clone of viral ORF for BcLF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401697

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BcLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101157 represents NCBI reference of YP_401697 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGCAACGAGGGTGTGGAAAACCGGCCATTTCCCTATCTGACTGTGGATGCTGACCTGTTGAGCA
  ATCTGAGACAGAGTGCTGCCGAGGGACTCTTTCACAGTTTTGATCTTTTGGTGGGCAAGGATGCGCGAGA
  GGCCGGTATAAAGTTTGAAGTCCTGCTGGGCGTCTACACAAATGCAATCCAGTATGTCCGATTCCTGGAG
  ACAGCACTGGCCGTTAGCTGCGTGAACACCGAGTTTAAAGACCTGAGTCGGATGACTGACGGAAAGATAC
  AGTTTCGCATCAGCGTGCCCACCATTGCCCATGGCGACGGTCGAAGACCCTCAAAACAGCGGACATTTAT
  AGTGGTAAAGAATTGCCATAAACATCATATCTCAACCGAGATGGAGTTGAGCATGCTGGATCTCGAGATT
  CTGCATAGTATCCCCGAGACCCCTGTGGAGTATGCCGAGTACGTAGGGGCAGTCAAGACAGTGGCCTCTG
  CACTGCAGTTCGGTGTGGATGCGCTTGAACGAGGTCTTATTAACACGGTGTTGTCTGTGAAACTGAGGCA
  TGCCCCCCCAATGTTTATCTTGCAGACCTTGGCTGACCCCACTTTTACTGAACGCGGATTTAGCAAAACA
  GTGAAGAGTGACCTGATCGCAATGTTCAAACGGCATCTGTTGGAACACTCATTCTTCCTCGACAGAGCTG
  AGAATATGGGAAGCGGCTTCAGTCAGTATGTGCGCTCCAGGCTCTCCGAAATGGTGGCTGCTGTGTCCGG
  GGAGTCCGTCCTGAAAGGCGTTAGCACCTACACCACTGCCAAGGGAGGTGAACCGGTGGGTGGAGTTTTC
  ATCGTGACAGACAACGTCCTCAGGCAGCTCCTGACTTTTCTGGGTGAGGAGGCTGACAATCAGATCATGG
  GTCCGTCATCATACGCATCCTTCGTGGTGAGGGGAGAGAACCTGGTCACAGCAGTGAGCTACGGTAGGGT
  GATGAGGACCTTTGAACACTTCATGGCAAGGATAGTTGATTCCCCTGAAAAAGCCGGATCAACTAAGAGC
  GATCTGCCTGCCGTCGCAGCCGGAGTTGAGGACCAACCTCGCGTTCCAATTTCTGCTGCCGTGATCAAGC
  TTGGCAACCACGCCGTAGCTGTGGAGTCTCTTCAGAAGATGTATAATGACACCCAATCACCATATCCACT
  GAATAGGCGGATGCAGTACTCATATTACTTTCCTGTAGGCCTGTTTATGCCCAATCCTAAATACACGACA
  AGTGCGGCTATCAAGATGTTGGATAATCCAACCCAACAACTCCCCGTGGAGGCCTGGATAGTAAACAAAA
  ACAATCTGCTGCTGGCATTCAACCTGCAGAATGCACTGAAAGTCCTGTGTCATCCACGACTGCACACTCC
  CGCTCATACACTCAATTCACTCAATGCGGCGCCTGCTCCTAGAGACCGGAGAGAGACCTATAGTCTTCAG
  CACCGCAGGCCAAACCACATGAATGTACTGGTGATCGTTGATGAGTTCTATGACAACAAATATGCTGCAC
  CAGTGACAGATATCGCACTCAAGTGCGGGCTCCCTACCGAAGACTTCTTGCACCCTAGTAACTATGACCT
  GCTTCGGTTGGAGCTGCATCCACTCTACGACATCTACATAGGACGGGACGCTGGGGAGAGAGCCCGCCAC
  AGAGCTGTCCATCGCCTGATGGTCGGGAACCTGCCAACTCCACTGGCCCCGGCAGCCTTTCAGGAGGCAA
  GGGGTCAGCAGTTTGAAACCGCAACTAGTCTCGCACATGTCGTTGATCAGGCCGTCATCGAAACCGTGCA
  GGACACAGCCTACGACACCGCATACCCAGCCTTTTTCTATGTGGTGGAGGCCATGATACACGGGTTCGAG
  GAAAAGTTCGTGATGAATGTGCCTCTGGTCAGCCTGTGCATAAACACTTATTGGGAGCGAAGCGGACGGC
  TCGCTTTCGTAAATTCATTTAGCATGATTAAATTTATTTGCCGGCACCTTGGCAACAATGCTATCAGCAA
  GGAGGCTTACTCTATGTACCGCAAGATTTATGGGGAGTTGATCGCTTTGGAGCAGGCTCTCATGAGACTT
  GCAGGATCCGATGTCGTGGGGGACGAGTCTGTAGGCCAATATGTATGTGCACTCCTGGATCCAAATCTGC
  TGCCCCCCGTAGCCTATACTGATATCTTCACTCACCTGCTCACGGTGAGTGACAGGGCGCCGCAAATTAT
  CATCGGGAATGAAGTCTATGCCGACACACTCGCTGCCCCGCAGTTCATTGAGCGCGTGGGCAACATGGAC
  GAGATGGCCGCCCAATTTGTCGCCCTGTACGGATACAGAGTGAACGGGGATCACGACCACGATTTCCGCC
  TGCACCTCGGTCCATACGTCGACGAGGGCCATGCAGATGTCCTGGAAAAAATTTTCTACTACGTGTTCCT
  CCCAACCTGTACCAATGCCCACATGTGTGGTCTGGGGGTGGATTTTCAGCATGTGGCTCAGACACTGGCT
  TACAACGGCCCCGCCTTCTCTCACCATTTCACCCGGGATGAAGATATTCTTGACAACCTTGAAAATGGAA
  CTCTGCGCGATCTTCTCGAGATCAGCGATCTCAGACCTACAGTTGGCATGATAAGAGATCTCTCCGCAAG
  CTTCATGACCTGCCCTACCTTCACGAGAGCCGTTAGAGTGAGCGTAGACAACGATGTCACACAGCAGCTT
  GCCCCTAACCCCGCCGACAAACGGACCGAGCAAACTGTGCTGGTCAACGGGCTCGTTGCCTTTGCATTTA
  GTGAACGAACCAGGGCGGTAACTCAGTGTCTGTTCCACGCTATACCTTTCCATATGTTCTATGGAGACCC
  CAGGGTAGCAGCCACGATGCACCAAGATGTCGCCACTTTCGTGATGAGGAACCCTCAACAGAGGGCAGTG
  GAAGCCTTCAACCGCCCAGAGCAACTTTTCGCCGAGTATCGGGAATGGCACCGCAGTCCGATGGGGAAGT
  ACGCGGCTGAGTGTCTGCCTTCCCTGGTGTCCATCAGCGGCATGACAGCCATGCACATAAAAATGTCCCC
  GATGGCCTATATCGCGCAGGCAAAATTGAAGATTCACCCAGGGGTTGCCATGACTGTCGTAAGAACAGAC
  GAAATTTTGTCCGAGAATATTCTGTTCAGCTCCAGAGCCTCTACCTCTATGTTTATTGGAACACCCAATG
  TCTCCCGGAGGGAAGCGCGGGTCGATGCTGTTACCTTCGAGGTGCACCATGAGATGGCCAGCATCGATAC
  AGGACTGAGCTATTCTAGTACAATGACACCTGCTAGGGTGGCTGCCATAACAACTGATATGGGAATTCAT
  ACCCAGGACTTTTTCAGCGTGTTCCCAGCAGAAGCATTCGGCAACCAGCAGGTGAATGACTATATCAAAG
  CCAAGGTTGGTGCCCAAAGGAACGGGACGCTCCTCAGAGATCCCCGGACCTACCTCGCCGGCATGACTAA
  CGTTAATGGAGCTCCGGGCCTTTGTCATGGGCAGCAAGCCACCTGCGAGATTATTGTGACCCCGGTGACT
  GCAGACGTGGCCTACTTTCAGAAATCCAATAGTCCTCGCGGCCGCGCGGCTTGCGTGGTTAGTTGCGAGA
  ATTACAACCAAGAGGTCGCTGAGGGTCTGATTTATGACCATAGCCGCCCAGACGCCGCGTATGAGTATAG
  AAGCACAGTGAATCCTTGGGCTTCACAGCTGGGTAGTCTTGGAGACATTATGTACAACTCTAGCTACCGC
  CAGACTGCTGTCCCAGGACTGTACAGCCCTTGTCGCGCCTTCTTCAACAAGGAAGAGCTGCTTAGAAATA
  ACAGAGGACTGTATAATATGGTCAATGAGTACAGTCAGAGATTGGGGGGTCACCCTGCCACTAGCAATAC
  AGAAGTGCAGTTCGTGGTGATCGCCGGAACGGATGTTTTTTTGGAACAACCATGCAGTTTCCTGCAGGAG
  GCCTTTCCCGCTCTGTCCGCCTCTTCCAGAGCGTTGATCGACGAATTCATGTCCGTTAAGCAGACCCACG
  CACCCATCCATTATGGCCATTATATTATCGAGGAGGTAGCTCCAGTTCGGCGAATACTCAAATTTGGCAA
  TAAAGTGGTGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101157 representing YP_401697
  Red=Cloning sites Green=Tags
  
  MASNEGVENRPFPYLTVDADLLSNLRQSAAEGLFHSFDLLVGKDAREAGIKFEVLLGVYTNAIQYVRFLE
  TALAVSCVNTEFKDLSRMTDGKIQFRISVPTIAHGDGRRPSKQRTFIVVKNCHKHHISTEMELSMLDLEI
  LHSIPETPVEYAEYVGAVKTVASALQFGVDALERGLINTVLSVKLRHAPPMFILQTLADPTFTERGFSKT
  VKSDLIAMFKRHLLEHSFFLDRAENMGSGFSQYVRSRLSEMVAAVSGESVLKGVSTYTTAKGGEPVGGVF
  IVTDNVLRQLLTFLGEEADNQIMGPSSYASFVVRGENLVTAVSYGRVMRTFEHFMARIVDSPEKAGSTKS
  DLPAVAAGVEDQPRVPISAAVIKLGNHAVAVESLQKMYNDTQSPYPLNRRMQYSYYFPVGLFMPNPKYTT
  SAAIKMLDNPTQQLPVEAWIVNKNNLLLAFNLQNALKVLCHPRLHTPAHTLNSLNAAPAPRDRRETYSLQ
  HRRPNHMNVLVIVDEFYDNKYAAPVTDIALKCGLPTEDFLHPSNYDLLRLELHPLYDIYIGRDAGERARH
  RAVHRLMVGNLPTPLAPAAFQEARGQQFETATSLAHVVDQAVIETVQDTAYDTAYPAFFYVVEAMIHGFE
  EKFVMNVPLVSLCINTYWERSGRLAFVNSFSMIKFICRHLGNNAISKEAYSMYRKIYGELIALEQALMRL
  AGSDVVGDESVGQYVCALLDPNLLPPVAYTDIFTHLLTVSDRAPQIIIGNEVYADTLAAPQFIERVGNMD
  EMAAQFVALYGYRVNGDHDHDFRLHLGPYVDEGHADVLEKIFYYVFLPTCTNAHMCGLGVDFQHVAQTLA
  YNGPAFSHHFTRDEDILDNLENGTLRDLLEISDLRPTVGMIRDLSASFMTCPTFTRAVRVSVDNDVTQQL
  APNPADKRTEQTVLVNGLVAFAFSERTRAVTQCLFHAIPFHMFYGDPRVAATMHQDVATFVMRNPQQRAV
  EAFNRPEQLFAEYREWHRSPMGKYAAECLPSLVSISGMTAMHIKMSPMAYIAQAKLKIHPGVAMTVVRTD
  EILSENILFSSRASTSMFIGTPNVSRREARVDAVTFEVHHEMASIDTGLSYSSTMTPARVAAITTDMGIH
  TQDFFSVFPAEAFGNQQVNDYIKAKVGAQRNGTLLRDPRTYLAGMTNVNGAPGLCHGQQATCEIIVTPVT
  ADVAYFQKSNSPRGRAACVVSCENYNQEVAEGLIYDHSRPDAAYEYRSTVNPWASQLGSLGDIMYNSSYR
  QTAVPGLYSPCRAFFNKEELLRNNRGLYNMVNEYSQRLGGHPATSNTEVQFVVIAGTDVFLEQPCSFLQE
  AFPALSASSRALIDEFMSVKQTHAPIHYGHYIIEEVAPVRRILKFGNKVVF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 4143 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401697
• RefSeq ORF: 4143 bp
• Locus ID: 3783688
• UniProt ID: P03211
• MW: 153.9 kDa