BALF4 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101173

Myc-DDK-tagged ORF clone of viral ORF for BALF4 [Human herpesvirus 4], codon optimized for human cell expression, YP_401713

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BALF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101173 represents NCBI reference of YP_401713 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACGAAGAAGAGTCCTGTCAGTTGTAGTGCTCCTGGCGGCGCTGGCATGTAGGCTGGGAGCCCAGA
  CCCCAGAGCAGCCCGCACCTCCTGCTACCACAGTGCAGCCTACAGCCACCAGGCAGCAAACTTCCTTCCC
  CTTCAGAGTGTGCGAGCTGTCTTCCCACGGTGACCTGTTCCGGTTTAGCTCTGATATTCAATGTCCCTCC
  TTCGGCACTAGGGAGAACCACACTGAAGGTTTGCTGATGGTGTTTAAAGATAACATCATTCCTTATAGTT
  TCAAGGTGAGGAGCTACACAAAGATCGTAACCAATATATTGATTTACAATGGCTGGTATGCTGATTCCGT
  GACTAACCGGCATGAAGAGAAGTTTAGTGTGGATTCATACGAGACAGACCAGATGGACACCATCTATCAG
  TGCTACAACGCTGTGAAGATGACAAAGGACGGCCTTACAAGGGTGTACGTGGACCGGGACGGAGTAAATA
  TCACCGTCAATCTTAAACCTACAGGAGGGTTGGCTAACGGCGTGCGGAGATATGCCTCACAAACCGAGCT
  GTACGATGCGCCGGGATGGCTGATCTGGACGTACCGCACCAGAACCACCGTGAATTGTCTCATCACTGAC
  ATGATGGCAAAGAGTAATAGCCCATTCGACTTCTTCGTCACCACTACAGGCCAGACTGTCGAAATGTCAC
  CATTCTACGATGGCAAAAATAAGGAAACATTTCATGAGCGCGCCGATAGTTTTCACGTTCGGACTAACTA
  CAAAATCGTGGATTACGATAATAGGGGCACGAACCCCCAGGGAGAGAGACGGGCCTTCCTCGACAAGGGC
  ACGTACACCCTGTCTTGGAAGCTGGAAAATCGGACCGCGTACTGTCCACTTCAGCACTGGCAGACCTTTG
  ACTCTACCATAGCTACAGAAACGGGTAAGTCCATCCACTTTGTTACCGATGAGGGAACATCTTCCTTTGT
  GACGAACACTACCGTCGGCATTGAGCTGCCGGACGCCTTTAAGTGTATTGAGGAGCAGGTGAACAAAACA
  ATGCATGAGAAGTATGAGGCTGTCCAGGACCGCTATACCAAGGGCCAGGAGGCCATTACATATTTTATAA
  CATCCGGAGGCCTGCTGCTCGCATGGCTCCCCCTCACCCCGAGATCCTTGGCTACGGTGAAGAACCTGAC
  AGAATTGACCACTCCAACTTCAAGCCCGCCTAGTTCCCCGTCTCCCCCCGCACCATCCGCAGCTCGGGGG
  TCAACCCCAGCGGCAGTACTGAGAAGGAGGCGGCGAGATGCTGGAAACGCAACAACCCCGGTTCCCCCAA
  CAGCCCCGGGAAAGTCCCTCGGGACCCTTAACAATCCCGCGACTGTGCAGATCCAGTTTGCATACGATAG
  CCTCCGCCGGCAAATAAATAGGATGCTGGGAGACCTGGCACGCGCGTGGTGTCTGGAGCAGAAGAGACAG
  AATATGGTGCTCCGGGAACTTACCAAGATAAATCCAACAACAGTAATGTCCAGCATCTATGGCAAAGCTG
  TCGCCGCAAAAAGATTGGGAGATGTCATAAGCGTAAGTCAGTGCGTGCCGGTGAACCAAGCTACAGTTAC
  TCTCAGAAAAAGTATGCGAGTTCCTGGCTCTGAAACGATGTGTTATTCACGCCCTTTGGTGAGCTTCAGC
  TTCATTAACGATACCAAGACCTACGAGGGCCAGCTGGGCACTGACAACGAGATATTTCTGACCAAAAAGA
  TGACCGAGGTGTGCCAAGCTACCTCCCAGTACTACTTCCAAAGCGGTAACGAAATCCATGTGTATAACGA
  CTATCATCACTTTAAAACGATCGAGCTCGACGGTATAGCAACCCTGCAAACGTTTATTAGCCTGAACACC
  AGCCTGATCGAAAATATCGATTTTGCCTCCCTCGAACTTTATAGCCGAGATGAGCAGAGAGCTTCCAATG
  TGTTTGATCTTGAGGGCATCTTTCGGGAGTACAATTTTCAGGCACAGAATATCGCTGGACTTAGGAAAGA
  TCTGGATAACGCCGTCTCTAATGGGAGGAACCAGTTTGTGGATGGCTTGGGAGAGCTGATGGATTCATTG
  GGCAGCGTCGGGCAGTCTATTACGAATTTGGTCAGTACAGTAGGCGGTTTGTTCTCATCCCTCGTTTCAG
  GGTTCATCTCATTTTTCAAGAACCCGTTCGGGGGAATGCTGATTCTGGTGCTCGTGGCTGGGGTCGTGAT
  CTTGGTAATAAGTCTTACTCGCAGAACCAGACAGATGTCCCAGCAGCCTGTTCAGATGCTGTATCCTGGC
  ATTGATGAATTGGCGCAGCAGCACGCTTCAGGGGAGGGGCCTGGTATCAATCCTATTTCAAAGACCGAGC
  TGCAAGCAATAATGCTGGCGCTGCACGAACAAAATCAAGAGCAGAAACGGGCTGCCCAGAGGGCCGCCGG
  ACCATCAGTCGCCAGTCGGGCTCTCCAAGCAGCCCGAGACAGATTCCCCGGACTGCGGAGGAGAAGGTAC
  CACGATCCTGAGACTGCTGCCGCTCTGCTGGGTGAAGCAGAAACTGAATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101173 representing YP_401713
  Red=Cloning sites Green=Tags
  
  MTRRRVLSVVVLLAALACRLGAQTPEQPAPPATTVQPTATRQQTSFPFRVCELSSHGDLFRFSSDIQCPS
  FGTRENHTEGLLMVFKDNIIPYSFKVRSYTKIVTNILIYNGWYADSVTNRHEEKFSVDSYETDQMDTIYQ
  CYNAVKMTKDGLTRVYVDRDGVNITVNLKPTGGLANGVRRYASQTELYDAPGWLIWTYRTRTTVNCLITD
  MMAKSNSPFDFFVTTTGQTVEMSPFYDGKNKETFHERADSFHVRTNYKIVDYDNRGTNPQGERRAFLDKG
  TYTLSWKLENRTAYCPLQHWQTFDSTIATETGKSIHFVTDEGTSSFVTNTTVGIELPDAFKCIEEQVNKT
  MHEKYEAVQDRYTKGQEAITYFITSGGLLLAWLPLTPRSLATVKNLTELTTPTSSPPSSPSPPAPSAARG
  STPAAVLRRRRRDAGNATTPVPPTAPGKSLGTLNNPATVQIQFAYDSLRRQINRMLGDLARAWCLEQKRQ
  NMVLRELTKINPTTVMSSIYGKAVAAKRLGDVISVSQCVPVNQATVTLRKSMRVPGSETMCYSRPLVSFS
  FINDTKTYEGQLGTDNEIFLTKKMTEVCQATSQYYFQSGNEIHVYNDYHHFKTIELDGIATLQTFISLNT
  SLIENIDFASLELYSRDEQRASNVFDLEGIFREYNFQAQNIAGLRKDLDNAVSNGRNQFVDGLGELMDSL
  GSVGQSITNLVSTVGGLFSSLVSGFISFFKNPFGGMLILVLVAGVVILVISLTRRTRQMSQQPVQMLYPG
  IDELAQQHASGEGPGINPISKTELQAIMLALHEQNQEQKRAAQRAAGPSVASRALQAARDRFPGLRRRRY
  HDPETAAALLGEAETEF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 2571 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401713
• RefSeq ORF: 2571 bp
• Locus ID: 3783680
• UniProt ID: P03211
• MW: 95.6 kDa