BSLF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101122

Myc-DDK-tagged ORF clone of viral ORF for BSLF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401662

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BSLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101122 represents NCBI reference of YP_401662 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCCCCCGTAGTAATCAAAGCTCTCGTAGCCAGCAATACTGACATAGCCGAAGCCATCCTGGACG
  CCATTCTGTCCAGGCCTGACGAAGGGTTTCGCCTCTTTTGCTTGTGCCATAACGCCAGTCCTCTCCACCA
  TGTGGCAGGGTCTCTTGTTGAACTGCAGCTGCATCTGCCAAAGAAAAGGCTGACCTCTCAGTCTCGCTGT
  GGGCTGGTGCTGACCCTGCACTTGCCAGCCGAGGAAGCCTTCCCATTCCTCCGCGGACTGACTCCCCTGA
  CCGCCGACAGGCTCTCCACATACCTTGATCGGGCAGGAGCACTGAGAAGCCTTACCCCACTTGTGGAACT
  GCTGACACTGTCCGCCAAAAAGCAGCCGCAGGGGGATGCCCGCGGAAGAGTGGCTTGGCTTCGCCCGAAG
  ATCGTGGGCTGCCTCCGCAGAATTTACCGGGTCAATATAAGTGCACGCTGGTTTATTTCCACTTTTGGCT
  CCCATGAGGCTCAGTTCGTTCTGGTGACCGCAGCCTACTACTTCTGGGGTATCCCTTGCACTATCGAGAC
  CCTGGCACATCTGACTGAGCTGTTTACTAGTGAGTCCGGTCAGTCCCTCGCAGCTGTTACTTCTCTGGCT
  GAATTGGGGGAAGTTTTCGGGTCCAGCGCCTGGGCCGAACAGACCGAAGCATTTGCACACTTCGCTCACG
  AGAAATTGCGCAGGGACTCCAGGGAGATCCGCGCCGTGGCTCGCACCATCGACGCCTACAGAGGACGGCT
  TCCTCTCGCATCTGCTGATCTGGTCCGGTATGTGTATCTCGCACATGCACAATGCTTTAACGAGGGGACC
  TTTAAGCGGTACAGCCAGCTGACTAGTATGGGAGAAATCGGCTGCCTCCCTTCCGGCGGGGTGGTGCTCC
  CGTCCCTGCTGGATCGAGGCTTCGCCGAGCACATGCGGACTTATTTTACCCGGGAGACGTACCTGGCAGA
  ACACGTTAGAGTGCAACAATTGAAAATCCGAATGGAACCGCCGGCCCCTTACACCTGGGATCCAGACCCT
  GATGACGGACTCATGCGGGCCTGGGCCGGGCTGAGCGTGGACGTGGCCCGGGAACTGGTTGAGCTGGCTA
  GGTGGCACGCAGACGAGGGACCCACCTATCCCCCAACTCTGCAGGGATTCCTCTGTTTGGCTGGCCAGGC
  AACATGTAGGGGGCAGTGGAACCCTAAGGAGCAATTCCTCCCCCCCACTGTCCTGAGAAGAGTTCAGAGG
  CTCCCTGTGTTTCTGTGTCACTTTGCCGACCGCCATTACTTCGTGATGACTGCTGCAGATCCCTTTAGCT
  CCCACCTGGCTGAAGTCGTCTCAACACCCACGAATTGTAGGCTTCCTGATACTTGCCTGACCCGAGCCCT
  GTCATATACTCCCGTGTACTACAGTCAGAATTCACTTTCAGAGCAGCTGTTTGTGTCTCGGCATGAGTAC
  TTCAATCCGCGGTTGCCCGTGTGTAATCTGGTGCTGGATCTGGACTTGAAGATAAAGGGGGCACCCTGGA
  GCCTCGAGGAAATTTACGACCTGTGTAGGACAGTGCGAAGGGAAGTCTTGCGGCTCATGCGACGCCTGGG
  ACCGGTAAGCCGAGCGCACCCTGTCTACTTCTTTAAATCTGCTTGCCCACCAGCTGATCCCGACAACATG
  GAAGACGTCCTGCCTTTTTGCATTTGTACCGGCAAGCTGGGATTCAGGGTGATCACCCCCTTGCCGCGAG
  GCCACGCAATCGTGGGCACAAGTGCAGTCCAGGGCTTCGTCAGTGTGCTGCAAAAGCTCATGGGCCTGAC
  AGCGTGCCTGAGACGAATGCGACACAAAATCAAGGAGATTGGGGCACCTCTCTTCGACAGCGGCGTTTAT
  CACGCCGGAAGATGTATACGCCTGCCCCATACGTACAAGGTGGACCGAGGCGGCGGCCTTTCAAGGCAAC
  TCAGATTGTTCGTTTGCCATCCCGAAGAGGAGGACAAGCACAGCTATGTGAAAAATGCGTTGAACATACA
  GAATCTCTTGCATCACTCTCTGCACGTGGGGTGGCCTGCCCCGAAGACATTCTGTTATCACATCGCGGAC
  GATGGAAGAGACTACTTGATCCAGAGGACTCGCGAGACCCTCCCCCCTACTGTGGAAAACGTGTGCGCGA
  TGATCGAAGGGCACCTGGGACTGGATTTGGTTGCCTGGGTGTCCAGCTGCATTTGGCCCTCCTTGATGTC
  CACTTTGGCTACAGCTGTGCCAGAAGACAAGTTTCCACAGTTCCTGCACGTGACATTCGAGCAAACTGGC
  CCTAACCTCGTGCAGGTGTGCCACGCCCGGGGCAGAAACTTCGCTTGCCTGAGACACACCCACCGCGCCA
  GCAGCAAAAACGTCAGAGTCTTTCTTGTGCTTTACTATACATCACAAGCAATCACGGTCACATTTATGTC
  TCAGTGTTTTGCTGGAAGATGCGGCGCTAATCAGCCCACAGCTCACTTTTCCATTAGCGTACCTGCCTCC
  CGGATCATTAACAGGGCGGAGGCGTCTCAGGACAGCACCACGTCTCAGCTGGCACGCAGGAGGGATAGAC
  AGGATGGCAGTTTTTCCGAAACACTGCCTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101122 representing YP_401662
  Red=Cloning sites Green=Tags
  
  MSAPVVIKALVASNTDIAEAILDAILSRPDEGFRLFCLCHNASPLHHVAGSLVELQLHLPKKRLTSQSRC
  GLVLTLHLPAEEAFPFLRGLTPLTADRLSTYLDRAGALRSLTPLVELLTLSAKKQPQGDARGRVAWLRPK
  IVGCLRRIYRVNISARWFISTFGSHEAQFVLVTAAYYFWGIPCTIETLAHLTELFTSESGQSLAAVTSLA
  ELGEVFGSSAWAEQTEAFAHFAHEKLRRDSREIRAVARTIDAYRGRLPLASADLVRYVYLAHAQCFNEGT
  FKRYSQLTSMGEIGCLPSGGVVLPSLLDRGFAEHMRTYFTRETYLAEHVRVQQLKIRMEPPAPYTWDPDP
  DDGLMRAWAGLSVDVARELVELARWHADEGPTYPPTLQGFLCLAGQATCRGQWNPKEQFLPPTVLRRVQR
  LPVFLCHFADRHYFVMTAADPFSSHLAEVVSTPTNCRLPDTCLTRALSYTPVYYSQNSLSEQLFVSRHEY
  FNPRLPVCNLVLDLDLKIKGAPWSLEEIYDLCRTVRREVLRLMRRLGPVSRAHPVYFFKSACPPADPDNM
  EDVLPFCICTGKLGFRVITPLPRGHAIVGTSAVQGFVSVLQKLMGLTACLRRMRHKIKEIGAPLFDSGVY
  HAGRCIRLPHTYKVDRGGGLSRQLRLFVCHPEEEDKHSYVKNALNIQNLLHHSLHVGWPAPKTFCYHIAD
  DGRDYLIQRTRETLPPTVENVCAMIEGHLGLDLVAWVSSCIWPSLMSTLATAVPEDKFPQFLHVTFEQTG
  PNLVQVCHARGRNFACLRHTHRASSKNVRVFLVLYYTSQAITVTFMSQCFAGRCGANQPTAHFSISVPAS
  RIINRAEASQDSTTSQLARRRDRQDGSFSETLPN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 2622 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401662
• RefSeq ORF: 2622 bp
• Locus ID: 3783730
• UniProt ID: P03211
• MW: 98.0 kDa