BGRF1/BDRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101150

Myc-DDK-tagged ORF clone of viral ORF for BGRF1/BDRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401690

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BGRF1/BDRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101150 represents NCBI reference of YP_401690 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTACGCTTCCCAGCGGGGCAGGCTGACCGAAAATCTCCGCAACGCCCTGCAGCAAGATTCCACAA
  CTCAGGGTTGTCTTGGAGCAGAAACTCCAAGCATCATGTACACTGGCGCCAAGAGCGATCGGTGGGCACA
  CCCCCTGGTTGGAACTATACACGCCAGTAACCTGTATTGCCCCATGCTTCGGGCCTACTGCCGCCATTAC
  GGCCCAAGGCCGGTTTTTGTCGCGTCAGACGAATCACTCCCGATGTTCGGAGCCTCTCCAGCTTTGCACA
  CCCCCGTTCAAGTCCAGATGTGCCTGCTGCCCGAATTGAGAGACACCCTGCAGCGACTGCTTCCCCCTCC
  AAACCTTGAGGATAGCGAGGCCCTCACAGAGTTTAAGACAAGTGTGAGCTCAGCACGGGCTATCCTGGAG
  GACCCCAACTTTCTTGAGATGAGAGAGTTCGTGACTTCCCTGGCTTCCTTCCTTAGTGGGCAATACAAAC
  ACAAACCCGCTCGACTGGAGGCATTCCAAAAGCAGGTGGTGCTCCATTCCTTTTACTTCCTTATTAGCAT
  TAAGTCTCTCGAAATAACAGACACTATGTTTGACATTTTCCAGTCTGCTTTCGGTCTGGAGGAAATGACC
  CTTGAAAAACTGCACATATTCAAGCAGAAGGCTTCTGTGTTTTTGATACCTAGACGCCATGGAAAGACGT
  GGATTGTCGTAGCCATAATTTCCCTGATCCTTTCAAATCTGTCTAATGTACAGATCGGGTACGTGGCCCA
  TCAAAAGCACGTGGCCAGTGCTGTCTTCACGGAAATCATTGATACTTTGACTAAGAGCTTTGACTCCAAG
  CGAGTGGAAGTAAACAAAGAGACTAGTACCATTACCTTCCGGCACAGCGGAAAGATATCTAGTACTGTCA
  TGTGCGCAACGTGTTTCAACAAAAACAGCATCCGCGGCCAAACATTCCATTTGTTGTTCGTGGATGAGGC
  AAATTTCATTAAAAAGGAGGCCCTGCCAGCAATATTGGGATTTATGCTGCAAAAGGACGCAAAGATTATC
  TTCATCTCTAGTGTTAACTCCGCCGATCAGGCCACCTCCTTTCTGTATAAACTGAAGGATGCACAGGAAA
  GGCTCTTGAACGTCGTCAGCTATGTGTGTCAAGAACACCGGCAGGACTTCGATATGCAGGACAGTATGGT
  GTCATGCCCTTGCTTCAGGCTGCACATCCCATCCTATATAACGATGGATTCTAACATAAGGGCGACTACC
  AATCTGTTTCTTGACGGCGCATTCAGCACCGAGCTCATGGGAGATACGTCTAGTTTGTCCCAAGGTAGCC
  TGTCACGGACGGTGAGAGACGATGCAATCAATCAACTTGAGCTTTGCCGCGTAGACACGCTGAATCCTCG
  CGTGGCCGGCAGGCTGGCCAGCAGTCTGTACGTTTATGTAGACCCTGCTTATACAAATAATACGTCCGCG
  AGCGGAACCGGGATTGCTGCCGTGACACATGACCGGGCTGACCCAAACCGAGTCATTGTATTGGGCCTGG
  AACACTTCTTTTTGAAAGACCTTACTGGTGATGCAGCCCTGCAGATCGCTACGTGCGTCGTGGCACTCGT
  GTCCAGTATTGTGACCCTGCACCCTCATTTGGAGGAGGTGAAAGTCGCTGTAGAGGGGAATTCCAGCCAG
  GATAGCGCCGTGGCAATTGCATCCATCATCGGTGAAAGCTGTCCTCTCCCTTGCGCCTTCGTTCACACCA
  AGGATAAGACCTCCTCTCTGCAGTGGCCGATGTATCTGCTCACTAACGAGAAAAGTAAAGCCTTTGAGAG
  ACTGATCTATGCCGTCAACACAGCTAGTCTCTCTGCCTCCCAGGTGACCGTGAGCAATACAATCCAGCTG
  TCATTCGACCCAGTGTTGTACCTGATCTCTCAGATCAGAGCCATCAAGCCTATCCCACTTCGAGACGGTA
  CCTATACATATACGGGGAAGCAGAGAAATCTTTCAGATGACGTACTGGTGGCACTGGTCATGGCACATTT
  TCTGGCCACCACCCAAAAGCATACATTCAAAAAAGTGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101150 representing YP_401690
  Red=Cloning sites Green=Tags
  
  MLYASQRGRLTENLRNALQQDSTTQGCLGAETPSIMYTGAKSDRWAHPLVGTIHASNLYCPMLRAYCRHY
  GPRPVFVASDESLPMFGASPALHTPVQVQMCLLPELRDTLQRLLPPPNLEDSEALTEFKTSVSSARAILE
  DPNFLEMREFVTSLASFLSGQYKHKPARLEAFQKQVVLHSFYFLISIKSLEITDTMFDIFQSAFGLEEMT
  LEKLHIFKQKASVFLIPRRHGKTWIVVAIISLILSNLSNVQIGYVAHQKHVASAVFTEIIDTLTKSFDSK
  RVEVNKETSTITFRHSGKISSTVMCATCFNKNSIRGQTFHLLFVDEANFIKKEALPAILGFMLQKDAKII
  FISSVNSADQATSFLYKLKDAQERLLNVVSYVCQEHRQDFDMQDSMVSCPCFRLHIPSYITMDSNIRATT
  NLFLDGAFSTELMGDTSSLSQGSLSRTVRDDAINQLELCRVDTLNPRVAGRLASSLYVYVDPAYTNNTSA
  SGTGIAAVTHDRADPNRVIVLGLEHFFLKDLTGDAALQIATCVVALVSSIVTLHPHLEEVKVAVEGNSSQ
  DSAVAIASIIGESCPLPCAFVHTKDKTSSLQWPMYLLTNEKSKAFERLIYAVNTASLSASQVTVSNTIQL
  SFDPVLYLISQIRAIKPIPLRDGTYTYTGKQRNLSDDVLVALVMAHFLATTQKHTFKKVH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 2070 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401690
• RefSeq ORF: 2070 bp
• Locus ID: 3783767
• UniProt ID: P03211
• MW: 76.7 kDa