BGLF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101152

Myc-DDK-tagged ORF clone of viral ORF for BGLF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401692

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BGLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101152 represents NCBI reference of YP_401692 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTACATATTGATAATCAGGTGCTGAGTGGGCTTGGGACCCCCCTGCTGGTGCACCTTTTCGTGC
  CCGACACGGTCATGGCCGAGTTGTGTCCTAACAGAGTGCCAAACTGCGAAGGAGCCTGGTGCCAAACTCT
  TTTTAGCGACAGAACTGGACTGACTCGGGTGTGCCGGGTTTTCGCCGCACGAGGCATGCTGCCGGGAAGG
  CCAAGCCATAGAGGAACCTTCACATCCGTGCCCGTATACTGCGACGAGGGACTCCCCGAGCTCTACAATC
  CCTTCCATGTCGCGGCCTTGCGGTTCTACGATGAGGGGGGTCTGGTGGGTGAGTTGCAGATATACTACCT
  GTCCCTCTTCGAGGGCGCCAAGAGGGCACTGACTGACGGTCATCTCATCCGGGAGGCTAGCGGGGTCCAG
  GAGAGCGCAGCCGCAATGCAGCCTATCCCTATCGATCCTGGACCCCCTGGAGGCGCAGGTATCGAACATA
  TGCCTGTCGCCGCTGCTCAAGTGGAGCATCCCAAGACCTACGACCTGAAACAGATCCTGCTGGAAATTAC
  TCAGGAGGAGAACAGGGGCGAGCAACGGTTGGGACACGCCGGTTCCCCTGCCCTGTGCCTGGGCCTTAGG
  CTGCGGGCAGGAGCAGAGACTAAAGCTGCTGCTGAAACCTCTGTGAGCAAACATCACCCCGCCCTGGAAA
  ACCCCAGCAACATCAGGGGTTCAGCTGGAGGAGAGGGGGGTGGCGGGCGAGCTGGTACAGGCGGAACAGT
  CGGAGTTGGAAGTGGGGCATTGAGCCGCGTCCCAGTGTCCTTCTCAAAAACAAGGCGGGCCATCAGAGAG
  TCCAGAGCCCTCGTAAGAGGCATCGCACATATCTTCTCACCACATGCCCTCTATGTGGTAACTTACCCCG
  AACTCAGTGCTCAGGGGAGGCTTCATAGGATGACAGCGGTCACCCATGCTAGCCCAGCAACAGACCTGGC
  TGAGGTAAGTATTCTTGGCGCCCCCGAAAGAGAGTTCCGGTTCCTGATCAGCGTAGCCTTGAGGATTTCT
  GCGAGCTTCAGAGAGAAATTGGCAATGCAGGCGTGGACCGCCCAGCAGGAGATTCCAGTGGTCATCCCAA
  CATCCTACTCACGCATTTATAAGAACTCTGACCTTATTCGCGAAGCGTTTTTCACAGTGCAGACCAGGGT
  AAGTTGGGAATCATGTTGGGTGAAGGCCACTATATCCAACGCCCCTAAGACTCCAGATGCCTGTCTGTGG
  ATTGATAGTCATCCCTTGTACGAGGAGGGTGCCAGTGCCTGGGGCAAAGTGATTGATAGTAGGCCCCCGG
  GAGGACTTGTTGGAGCTGCCTCTCAATTGGTCGCCCTGGGCACAGATGGCCATTGTGTGCATCTCGCCAC
  AACCAGTGATGGTCAGGCGTTTCTCGTGCTCCCCGGGGGGTTCGTGATCAAAGGACAACTGGCGTTGACC
  CCTGAGGAGAGGGGCTACATCCTCGCTCGCCACGGAATTAGACGGGAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101152 representing YP_401692
  Red=Cloning sites Green=Tags
  
  MDVHIDNQVLSGLGTPLLVHLFVPDTVMAELCPNRVPNCEGAWCQTLFSDRTGLTRVCRVFAARGMLPGR
  PSHRGTFTSVPVYCDEGLPELYNPFHVAALRFYDEGGLVGELQIYYLSLFEGAKRALTDGHLIREASGVQ
  ESAAAMQPIPIDPGPPGGAGIEHMPVAAAQVEHPKTYDLKQILLEITQEENRGEQRLGHAGSPALCLGLR
  LRAGAETKAAAETSVSKHHPALENPSNIRGSAGGEGGGGRAGTGGTVGVGSGALSRVPVSFSKTRRAIRE
  SRALVRGIAHIFSPHALYVVTYPELSAQGRLHRMTAVTHASPATDLAEVSILGAPEREFRFLISVALRIS
  ASFREKLAMQAWTAQQEIPVVIPTSYSRIYKNSDLIREAFFTVQTRVSWESCWVKATISNAPKTPDACLW
  IDSHPLYEEGASAWGKVIDSRPPGGLVGAASQLVALGTDGHCVHLATTSDGQAFLVLPGGFVIKGQLALT
  PEERGYILARHGIRREQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401692
• RefSeq ORF: 1521 bp
• Locus ID: 3783702
• UniProt ID: P03211
• MW: 54.5 kDa