BRRF2 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101136

Myc-DDK-tagged ORF clone of viral ORF for BRRF2 [Human herpesvirus 4], codon optimized for human cell expression, YP_401676

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101136 represents NCBI reference of YP_401676 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGACAACAGCGCGGCAGTGTAATTCTGGTACCCGAGCACCTTGCAGGCGCCCTTACTAAACTCA
  TGTCTGATTTTATCACGGGCCAAGATGTCACGTTGTCAGGGGGGAATATTGCTGTGAAGATCCGAGATGC
  CATCAACCAGACTCCGGGCGGGGGCGATGTGGCAATTCTGTCTAGTCTCTTTGCCCTTTGGAATGCCCTG
  CCTACCTCCGGCCGGCAGTCCTCCAGAGACGATCTCATTCCTGCCGCCGTCCAGGCTTTGACAACAGCTC
  ACAACCTGTGTCTGGGCGTGATCCCAGGCGAAACCTCTCACAAGGACACGCCTGAGAGCCTGCTCAGGGC
  CATCGTTACCGGGCTGCAGAAACTGTGGGTGGACAGCTGTGGCTGCCCGGAGTGTTTGCAGTGCCTGAAG
  GGTCTGAAGGCTATCAAACCTGGGCTCTATGAAATCCCGCGGATCATCCCACATACGAAGCAGTGCTCTC
  CAGTCAATCTCCTCAACATGCTTGTTCACAAACTCGTGGCCCTGCGAGGCCACGTGCAGCTCGCTTATGA
  CGCAAGGGTACTGACCCCTGACTTCCATGAGATCCCCGATCTGGACGACTCAGACGCAGTCTTCGCTAGA
  ACCCTGTTGGCAGCACTTTTTCACCTGAACATGTTCTTTATTCTCAAAGACTACATTACCCAGGACTCAA
  TGTCTCTTAAGCAGGCTCTCTCAGGGCACTGGATGTCTGCAACAGGAAACCCCCTGCCTGCAGCCCCAGA
  GACACTGCGGGACTATCTGGAAGCCTTCAGGAATTCAGACAACCACTTCTACCTGCCTACCACCGGGCCA
  CTTAACACTTTTCAGTTCCCTGAGGAGCTCTTGGGCCGCGTCGTGGTAATCGACTCCTCCTTGTGCGCAG
  CCTCCCACGTTCAGGACGTTATAACTCACGGTGTGGGAGCCGGGGTTCCAAGACCTCGGTTTAGTGCTCT
  TCCACCCGCCCCCAGCCGCGAGCCCCAGCAGACCTGTAGCCAACTGACATCAAGAGGCAACGAAAGTTCC
  AGAAGGAACCTGGGACAGCCCGGCGGTACCTCCCCGGCAGTCCCTCCCGTCTGCCCGATTGTCTCTCTCA
  CCGCTTCCGGAGCCAAACAGAATCGCGGGGGCATGGGATCACTCCATCTGGCAAAACCTGAAGAAACTAG
  CCCAGCAGTGTCCCCTGTATGTCCAATTGCTTCCCCGGCCGCCAGTCGATCCAAGCAGCATTGTGGCGTG
  ACAGGCAGCTCCCAGGCGGCCCCAAGTTTCTCTTCAGTGGCTCCTGTGGCATCACTGTCTGGCGATCTCG
  AAGAAGAAGAAGAAGGTTCCAGAGAGTCTCCTTCCCTCCCGTCCAGTAAAAAGGGAGATGAGGAGTTCGA
  AGCATGGCTCGAGGCTCAGGATGCCAACCTTGAAGATGTGCAAAGAGAGTTTTCCGGACTTCGGGTGATT
  GGAGACGAGGATGAGGATGGCAGCGAAGACGGCGAGTTCAGCGACCTCGACCTGAGTGACTCTGATCATG
  AGGGCGATGAGGGCGGGGGTGCAGTAGGAGGCGGCCGGTCCTTGCACTCTTTGTATAGCCTGTCTGTGGT
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101136 representing YP_401676
  Red=Cloning sites Green=Tags
  
  MSGQQRGSVILVPEHLAGALTKLMSDFITGQDVTLSGGNIAVKIRDAINQTPGGGDVAILSSLFALWNAL
  PTSGRQSSRDDLIPAAVQALTTAHNLCLGVIPGETSHKDTPESLLRAIVTGLQKLWVDSCGCPECLQCLK
  GLKAIKPGLYEIPRIIPHTKQCSPVNLLNMLVHKLVALRGHVQLAYDARVLTPDFHEIPDLDDSDAVFAR
  TLLAALFHLNMFFILKDYITQDSMSLKQALSGHWMSATGNPLPAAPETLRDYLEAFRNSDNHFYLPTTGP
  LNTFQFPEELLGRVVVIDSSLCAASHVQDVITHGVGAGVPRPRFSALPPAPSREPQQTCSQLTSRGNESS
  RRNLGQPGGTSPAVPPVCPIVSLTASGAKQNRGGMGSLHLAKPEETSPAVSPVCPIASPAASRSKQHCGV
  TGSSQAAPSFSSVAPVASLSGDLEEEEEGSRESPSLPSSKKGDEEFEAWLEAQDANLEDVQREFSGLRVI
  GDEDEDGSEDGEFSDLDLSDSDHEGDEGGGAVGGGRSLHSLYSLSVV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401676
• RefSeq ORF: 1611 bp
• Locus ID: 3783729
• UniProt ID: P03211
• MW: 57.0 kDa