BcRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101158

Myc-DDK-tagged ORF clone of viral ORF for BcRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401698

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BcRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101158 represents NCBI reference of YP_401698 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACAGGGAAAGCGCGAGATGGGCGGTGGACTGGAGGGCTTCTCCTCTCAGCTCGGACTGTTTTATG
  CCCTGGCCTGTAATCGATCCCCTCCCACTTTGCCTGAGGACGCCACTCTGCTCATAAAGTGGCTCGACAC
  AGCCTTGGGAAGGGAGGCTACATTTTATGCCTGCCGGGCCATGAGAAGACTTCTTCTGGGCGTTATTAGG
  ATGAACGACTGTCAAGAGCTTCCCCCCGGACTGATTATTCTCTCCCCTGGCACAGTCCCTGGCCCTCTGG
  GGGTACAATCCCTCGAACATACAGATTGTGAGATCTGGTCTAGCGCACACCCAGACCATGCCGCACACCT
  GCCAGTGCCAAGGGTCATTACATACACCGATTGCCCGGGGTCTATAAATACCTCTAGCATGTTCAGACTG
  ATTATCAGATACCTGAGCCACCATCAGTTCGAACGGTGCTTCGAGCAGTTCTGCAGGGTCGTTCCACGAA
  GATTCCTGGGGACATGTAAACGCAACTCCGCCAAGATGCTGGCTCATCTTAACCAGGTTACCAGAATACC
  ACCGTGTCCACCCTTTAGCGGAAGAGAGGCCCGGTTGAAGTTTCACTTTTTCAGTTGGTCAACTTTTATG
  CTCAGCTGGCCAAATAATGCGACACTTCGGGAGATCCGAACAAGGGCAGCCACCAACCTCACACATCATC
  CCCACCTCGTCGACACACTCTACCACGCTTCTCCTCAGACTCCCTTCCTCACCCGCAGCGGGGCGCTGTA
  TAGGTTCGTTACCTGTTGTAACTGTACGCTCCCGAACATCTCAATCCAGCAGTGCAAGGCCGGCGACCGC
  CCAGGCGACTTGGAAATCATTCTGCAGTCCAACGGAGGTGGTCGACCTGCCTCCTTCCAGTTTCCTTCCT
  CTCCCACCGGGTCTCTGCTGAGGTGTATAGTGGCCGCCTCCCTGCTCCCAGAAGTATCTGTAGGACATCA
  AGAGCTCAGCCCCCTGCGGTCCAGAAGCCAGGGGGGCCAGACGGACGTGCGGAGCGGACCCGATCCTGCT
  CGGAGACTCGTGGCTCTTCTGAGAAGGGAGGATGGCGCCCCCAAGGACCCTCCACTTGGACCCTTCGGAC
  ACCCGCGAGGTCCCGGCCCCGCAAAATCTGAAGACGAGGAGAGCGAGAGGAGGGATGCACCCCCACCTCC
  ACTGGACTCTAGTTTCCAAGCATCACGGCTGGTTCCCGTGGGTCCCGGTTTCCGGCTTCTGGTTTTCAAC
  ACAAACCGCGTAATCAACACTAAGCTTGTATGTTCTGAACCACTCGTGAAAATGAGGGTTTGCAATGTCC
  CCCGATTGATTAACAATTTCGTGGCACGAAAGTATGTTGTGAAAGAAACCGCTTTTACTGTGTCTCTCTT
  CTTCACAGATGGGGTAGGGGCAAACCTGGCCATCAACGTCAACATCTCAGGCACCTACCTGAGCTTTCTG
  CTGGCAATGACAAGCCTCCGCTGTTTCCTGCCTGTCGAGGCTATTTATCCTGCAGCCGTAAGTAACTGGA
  ATTCTACTCTTGATCTTCATGGACTGGAAAACCAGTCTCTGGTGAGAGAAAACAGATCAGGCGTGTTCTG
  GACAACAAACTTCCCCTCTGTGGTTAGTTGTCGGGACGGACTGAATGTGAGCTGGTTTAAGGCAGCCACC
  GCAACTATCTCCAGGGTTCACGGCCAAACATTGGAGCAGCACTTGATAAGAGAGATTACACCTATTGTCA
  CACACCGCGAAGCAAAAATTAGCCGCATAAAGAATAGACTGTTTACCCTCCTCGAGCTCAGAAATCGGAG
  CCAAATTCAGGTTCTGCATAAACGCTTCCTGGAGGGACTTCTCGACTGCGCCAGTCTTCTGCGGCTCGAT
  CCCAGCTGTATTAATAGAATTGCCAGCGAAGGACTCTTTGACTTCTCAAAACGCTCTATTGCCCATAGCA
  AAAATCGCCATGAATGTGCCCTCCTGGGACATCGCCACTCAGCCAATGTTACAAAGCTGGTCGTCAATGA
  GAGGAAGACAAGATTGGATATACTTGGACGGAATGCCAATTTTCTGACTCGCTGTAAGCATCAAGTGAAT
  TTGCGCCAGAGTCCTATCTTCCTCACTTTGCTGAGGCATATCAGGCGGCGCCTCGGCCTGGGACGAGCAT
  CTGTGAAGCGAGAAATCACCCTCCTCCTGGCACACCTGCGCAAGAAAACTGCACCTATCCATTGTCGAGA
  TGCTCAAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101158 representing YP_401698
  Red=Cloning sites Green=Tags
  
  MTQGKREMGGGLEGFSSQLGLFYALACNRSPPTLPEDATLLIKWLDTALGREATFYACRAMRRLLLGVIR
  MNDCQELPPGLIILSPGTVPGPLGVQSLEHTDCEIWSSAHPDHAAHLPVPRVITYTDCPGSINTSSMFRL
  IIRYLSHHQFERCFEQFCRVVPRRFLGTCKRNSAKMLAHLNQVTRIPPCPPFSGREARLKFHFFSWSTFM
  LSWPNNATLREIRTRAATNLTHHPHLVDTLYHASPQTPFLTRSGALYRFVTCCNCTLPNISIQQCKAGDR
  PGDLEIILQSNGGGRPASFQFPSSPTGSLLRCIVAASLLPEVSVGHQELSPLRSRSQGGQTDVRSGPDPA
  RRLVALLRREDGAPKDPPLGPFGHPRGPGPAKSEDEESERRDAPPPPLDSSFQASRLVPVGPGFRLLVFN
  TNRVINTKLVCSEPLVKMRVCNVPRLINNFVARKYVVKETAFTVSLFFTDGVGANLAINVNISGTYLSFL
  LAMTSLRCFLPVEAIYPAAVSNWNSTLDLHGLENQSLVRENRSGVFWTTNFPSVVSCRDGLNVSWFKAAT
  ATISRVHGQTLEQHLIREITPIVTHREAKISRIKNRLFTLLELRNRSQIQVLHKRFLEGLLDCASLLRLD
  PSCINRIASEGLFDFSKRSIAHSKNRHECALLGHRHSANVTKLVVNERKTRLDILGRNANFLTRCKHQVN
  LRQSPIFLTLLRHIRRRLGLGRASVKREITLLLAHLRKKTAPIHCRDAQV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 2250 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401698
• RefSeq ORF: 2250 bp
• Locus ID: 3783690
• UniProt ID: P03211
• MW: 83.8 kDa