BBLF4 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101141

Myc-DDK-tagged ORF clone of viral ORF for BBLF4 [Human herpesvirus 4], codon optimized for human cell expression, YP_401681

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BBLF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101141 represents NCBI reference of YP_401681 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAGAACCTCGCGCCCCCGAAGCCCTGAGCTCCACATTTATGCTCAATATGACTAGCGATGCTT
  CAGTCAGGAGGATTGTTCGGAGGATCGGGACCTTGGCTCGCAGAAGGGTGCAACAGCTGCCGGACATGGA
  GACGTTCTCCCCTGAATTCGACCCCGAACTTAGCGAACCCCCCTTCCTGCCTTTTAGCGCCTACGTCATT
  ACCGGAACCGCTGGCGCTGGAAAGAGCACGTCTGTTTCATGTTTGCACCACACGATGGACTGTCTCGTAA
  CCGGCGCTACCACAGTAGCTGCCCAGAACCTCTCACAGACCCTGAGGGCATACTGTCCCACAGTCTACTC
  CGCATTTGGGTTTAAAAGCCGGCATATCAACATGACTCAAAGGGTATCTTCTCACGGCCGGAGCACAGAT
  GCCGCGCTTGAAGAGCTCCAACGGAGAGACCTGGCCAAATATTGGCCTGTACTGAGTGACATCGCTGCGG
  AGTTCAGGCGGACCAAACCAAGGGGCCTTTATAGTGGGGTATCTGGACCCGCTTTCGAAGTTCTCAGGGA
  CATGCATCAAGGCCAGCTTTGGACAACAAATGTAATTGTGGTAGATGAGGCTGGTACCCTCTCCGTACAC
  ATACTGACAGCAGTGGTATTTTGTTACTGGTTTTTCAACGCCTGGCTGAGAACACCCCTCTACCGACGAG
  GAAGGATCCCCTGTATCGTTTGCGTGGGCAGCCCTACACAAACTGACGCCTTCCAGAGCAGTTTTAGCCA
  CGAGACGCAGGTTAACAAGATACGAGAATGTGATAATATTCTGACATTCCTGGTGGGCAACCCCCGGGCA
  GCAACCTACGTGGATGTGGCTAGGAATTGGGCTCTGTTCATCAATAACAAACGGTGCACTGATGTTCAGT
  TTGGACACCTGATGAAAACTCTGGAATATGGGCTGGAGCTCAGCCCCGACATTTTGGCGTACGTGGACCG
  ATTTGTGGTCCCACGCGCAGCTATCATGGATCCAGCACAGTACGTAGGTTGGACCAGACTGTTTTTGTCC
  CATGCCGAAGTTAAAACATTCCTCACCACTCTGCATGCTACACTTAAGACCGCCGGTCAGGGACGCGCTG
  CTAGGGGGACAGGTGGCGACGGGGGAGGCGTTACAATGTTTACCTGCCCTGTTGAGTGCGAAGTCTTCCT
  GGATCCTCTGGCTCAATATAAAACCTTGGTTGGCCTCCCCGGTTTGACTGCACATACCTGGTTGCAGAAA
  AATTATGCCCGGCTGGGAAATTACAGCCAATTCGCCGATCAAGATATGGTCCCAGTGGGCACCGAGCAGG
  ATGAAGAACGGGTGAAAGTCACATACAACGTAACCTACGTTAAACATAGTAGCGTGTCAGTGAACTGTAA
  AACCAAAAAGTCCATCTGCGGCTATACCGGTACATTTGGGGATTTCATGGATACTTTGGAAGCCGACAGT
  TTCGTTGAGGCACATGGTCATGAACAGCCCGAGTATGTCTACAGCTTTCTCGCCCGGCTGATTTACGGCG
  GCATTTACGCTTTCTCACACGGGGGTCATAGTCTGTGCGAGAATGGCGAGTACGTGGCAGAGCTGGGAGC
  CGTTCCACTGCCCGGGCGGACCTGGGACCCTGAAGTGACCGCTGGAATGGAACTGGGAGAGTTGCCTCTG
  GAGGTGGCATGGGACGGGGAGAGGTCTCCAGCGGCGGTGTTCTATGCTCGGGTGCTGGCTCCACCTGCCG
  CGAATTCCGCACCGCTCTGCAGTCTGCTTAACATCTACAACGATCTGAGGGCTTACTTTCGGCAGTGTCT
  CGATGTTGCAGTCCGGTACGGCGGGCGGGAGTTTAGGGACCTCCCCTTCTGTACCTTCACTAACAACATG
  CTCATCCGCGATAACATCGAATTCACTTCCGATGAACCACTGCTGCACGGGCTTCTGGACTATGCCAGTA
  CTACTGAGAATTACACCCTCCTGGGTTACACCCACCTGAACGTATTCTTTGGGATCAGGGGGAAACAGCA
  GCCACAGGATGCCGGGTCCAGTCGGATGCCCCGGCTGATGGTGAAGGATGAAGCCGGGTTTGTGTGCTGC
  CTGGAGCACAATACGAACAAGTTGTATGAGACCATCGAGGACAAAAGCCTCAACCTGTGCTCTATTAGGG
  ACTACGGCATATCTTCCAAATTGGCCATGACCATCGCCAAGGCACAGGGCCTGAGCCTGAATAAGGTGGC
  AATCTGTTTTGGCTCTCACAGAAACATTAAACCAGGCCACGTATATGTCGCCCTGTCCAGGGCGCGGCAC
  TCCAACTGTGTGGTAATGGACCGGAATCCTTTGTCCGAAATGATCACCGGAGAAGGAAACCCCGCCAGTG
  GATACATCGTGGACGCCTTGAAGAATTCTAGGGCTCTGCTCGTTTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101141 representing YP_401681
  Red=Cloning sites Green=Tags
  
  MAEEPRAPEALSSTFMLNMTSDASVRRIVRRIGTLARRRVQQLPDMETFSPEFDPELSEPPFLPFSAYVI
  TGTAGAGKSTSVSCLHHTMDCLVTGATTVAAQNLSQTLRAYCPTVYSAFGFKSRHINMTQRVSSHGRSTD
  AALEELQRRDLAKYWPVLSDIAAEFRRTKPRGLYSGVSGPAFEVLRDMHQGQLWTTNVIVVDEAGTLSVH
  ILTAVVFCYWFFNAWLRTPLYRRGRIPCIVCVGSPTQTDAFQSSFSHETQVNKIRECDNILTFLVGNPRA
  ATYVDVARNWALFINNKRCTDVQFGHLMKTLEYGLELSPDILAYVDRFVVPRAAIMDPAQYVGWTRLFLS
  HAEVKTFLTTLHATLKTAGQGRAARGTGGDGGGVTMFTCPVECEVFLDPLAQYKTLVGLPGLTAHTWLQK
  NYARLGNYSQFADQDMVPVGTEQDEERVKVTYNVTYVKHSSVSVNCKTKKSICGYTGTFGDFMDTLEADS
  FVEAHGHEQPEYVYSFLARLIYGGIYAFSHGGHSLCENGEYVAELGAVPLPGRTWDPEVTAGMELGELPL
  EVAWDGERSPAAVFYARVLAPPAANSAPLCSLLNIYNDLRAYFRQCLDVAVRYGGREFRDLPFCTFTNNM
  LIRDNIEFTSDEPLLHGLLDYASTTENYTLLGYTHLNVFFGIRGKQQPQDAGSSRMPRLMVKDEAGFVCC
  LEHNTNKLYETIEDKSLNLCSIRDYGISSKLAMTIAKAQGLSLNKVAICFGSHRNIKPGHVYVALSRARH
  SNCVVMDRNPLSEMITGEGNPASGYIVDALKNSRALLVY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 2427 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401681
• RefSeq ORF: 2427 bp
• Locus ID: 3783685
• UniProt ID: P03211
• MW: 89.9 kDa