BGLF4 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101148

Myc-DDK-tagged ORF clone of viral ORF for BGLF4 [Human herpesvirus 4], codon optimized for human cell expression, YP_401688

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BGLF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101148 represents NCBI reference of YP_401688 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGAATATGGCTGCCGAGCTGTCACCGACCAACTCAAGCTCAAGCGGAGAATTGTCCGTGTCCC
  CAGAACCTCCGAGGGAGACTCAGGCCTTCCTGGGTAAAGTTACCGTAATCGATTACTTTACGTTTCAACA
  TAAACACCTCAAGGTGACGAATATCGATGATATGACCGAGACTCTGTATGTGAAACTGCCTGAGAATATG
  ACTCGCTGTGATCACCTGCCAATCACCTGCGAATATCTTTTGGGAAGGGGAAGCTATGGTGCCGTTTACG
  CCCACGCCGACAACGCAACCGTGAAATTGTACGACAGCGTCACAGAGCTGTATCATGAACTCATGGTGTG
  TGACATGATTCAGATCGGTAAAGCCACAGCAGAGGACGGCCAAGACAAAGCCCTCGTTGATTACCTCTCT
  GCCTGTACTAGCTGCCATGCGTTGTTCATGCCACAGTTCCGGTGCTCTCTCCAAGACTATGGCCATTGGC
  ACGATGGCAGCATCGAGCCACTCGTGAGGGGTTTCCAGGGCCTCAAGGATGCAGTGTACTTTCTGAATAG
  ACATTGCGGCTTGTTTCACTCCGACATCTCACCTTCTAATATCCTGGTGGATTTCACAGATACTATGTGG
  GGCATGGGGAGGTTGGTGCTGACCGACTACGGCACCGCTTCACTCCACGACAGGAATAAGATGCTCGACG
  TGAGACTGAAAAGCAGTAAGGGGCGGCAGCTTTACAGATTGTACTGTCAACGCGAGCCATTTAGTATCGC
  GAAAGACACATATAAACCTCTGTGCCTCTTGTCCAAATGTTATATATTGAGGGGAGCAGGACATATTCCT
  GACCCTTCAGCATGCGGCCCGGTGGGTGCGCAGACCGCCCTGCGGCTGGACCTGCAGAGTCTCGGTTACA
  GCCTGCTCTACGGCATCATGCACCTGGCTGATTCCACACATAAAATACCTTACCCAAATCCCGACATGGG
  TTTCGATCGATCAGATCCGCTTTATTTTCTGCAGTTTGCTGCGCCGAAAGTGGTCCTGCTCGAGGTGCTC
  TCACAAATGTGGAACCTGAACCTGGACATGGGCCTGACATCATGCGGAGAAAGTCCCTGCGTCGACGTCA
  CCGCCGAGCACATGAGCCAATTTCTTCAGTGGTGTAGATCTCTGAAAAAAAGATTTAAAGAAAGCTATTT
  TTTTAACTGTAGGCCGCGATTCGAACACCCACATCTTCCCGGCCTGGTTGCAGAGCTTCTGGCCGACGAC
  TTTTTTGGACCAGACGGTCGGAGAGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101148 representing YP_401688
  Red=Cloning sites Green=Tags
  
  MDVNMAAELSPTNSSSSGELSVSPEPPRETQAFLGKVTVIDYFTFQHKHLKVTNIDDMTETLYVKLPENM
  TRCDHLPITCEYLLGRGSYGAVYAHADNATVKLYDSVTELYHELMVCDMIQIGKATAEDGQDKALVDYLS
  ACTSCHALFMPQFRCSLQDYGHWHDGSIEPLVRGFQGLKDAVYFLNRHCGLFHSDISPSNILVDFTDTMW
  GMGRLVLTDYGTASLHDRNKMLDVRLKSSKGRQLYRLYCQREPFSIAKDTYKPLCLLSKCYILRGAGHIP
  DPSACGPVGAQTALRLDLQSLGYSLLYGIMHLADSTHKIPYPNPDMGFDRSDPLYFLQFAAPKVVLLEVL
  SQMWNLNLDMGLTSCGESPCVDVTAEHMSQFLQWCRSLKKRFKESYFFNCRPRFEHPHLPGLVAELLADD
  FFGPDGRRG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401688
• RefSeq ORF: 1287 bp
• Locus ID: 3783704
• UniProt ID: P03211
• MW: 48.4 kDa