BaRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101118

Myc-DDK-tagged ORF clone of viral ORF for BaRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401656

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BaRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101118 represents NCBI reference of YP_401656 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAACTGCTGTATGTGCGCGACCATGAGGGCTTTGCATGCCTTACCGTGGAAACTCATCGCAATC
  GCTGGTTCGCAGCTCACATTGTCCTTACTAAGGATTGCGGATGCCTGAAACTTCTCAACGAAAGGGACCT
  GGAGTTCTACAAGTTTTTGTTCACATTTCTGGCAATGGCGGAAAAATTGGTGAATTTTAATATCGATGAA
  CTCGTGACGTCCTTTGAGAGCCATGACATCGATCATTACTATACTGAACAGAAAGCTATGGAGAACGTCC
  ACGGTGAGACATATGCTAACATCTTGAATATGTTGTTTGATGGGGATAGAGCAGCGATGAATGCCTACGC
  GGAGGCAATCATGGCCGACGAAGCACTCCAGGCTAAGATCAGCTGGCTGAGAGATAAGGTGGCCGCCGCT
  GTTACACTTCCCGAGAAAATACTGGTATTCTTGCTGATTGAGGGCATTTTTTTTATTAGCAGCTTTTACA
  GCATAGCCTTGCTTCGGGTTCGAGGACTCATGCCCGGGATTTGCCTGGCCAATAATTACATTAGCCGCGA
  TGAGTTGTTGCACACCAGGGCCGCCTCACTCCTGTACAACTCAATGACTGCAAAAGCCGACAGGCCTCGC
  GCCACTTGGATCCAGGAGTTGTTTAGGACTGCTGTGGAGGTGGAGACGGCCTTTATCGAGGCTCGGGGGG
  AAGGCGTCACCTTGGTGGACGTCCGAGCCATCAAACAGTTTCTCGAGGCCACCGCTGACCGCATCCTGGG
  AGATATTGGTCAAGCCCCACTGTATGGCACCCCCCCACCCAAGGATTGCCCACTCACGTACATGACGAGC
  ATCAAGCAGACAAACTTTTTCGAGCAGGAGTCCTCAGACTATACAATGTTGGTTGTGGACGATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101118 representing YP_401656
  Red=Cloning sites Green=Tags
  
  MSKLLYVRDHEGFACLTVETHRNRWFAAHIVLTKDCGCLKLLNERDLEFYKFLFTFLAMAEKLVNFNIDE
  LVTSFESHDIDHYYTEQKAMENVHGETYANILNMLFDGDRAAMNAYAEAIMADEALQAKISWLRDKVAAA
  VTLPEKILVFLLIEGIFFISSFYSIALLRVRGLMPGICLANNYISRDELLHTRAASLLYNSMTAKADRPR
  ATWIQELFRTAVEVETAFIEARGEGVTLVDVRAIKQFLEATADRILGDIGQAPLYGTPPPKDCPLTYMTS
  IKQTNFFEQESSDYTMLVVDDL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 906 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401656
• RefSeq ORF: 906 bp
• Locus ID: 3783683
• UniProt ID: P03211
• MW: 34.4 kDa